小鹅瘟LAMP检测方法的建立与应用以及无锡地区小鹅瘟流行状况调查.pdfVIP

崔洪、h小鹅瘟LAMP检测方法的建、)=和应用以及无锡地区小鹅瘟流行状况调查 Development and application LAMP method on detection of gosling plague VIrus ancl eDlOemlolOg；lcal mvestlgations ot擘；OSllng一? - 一 一 一一 plague in Wuxi area 一 一 Master candidate：Cui Hongping Adviser：Professor Qin Aij ian Professor Zhao Zhongxing Abstract：The gosling plague is a kind of acute and high—contacted contagion，which infacted gosling，with high mortality．This study firstly applied the loop—mediated isothermal amplification(LAMP)to the rapid detection of gosling plague．According to Gosling plague virus(GPV)genome，four specific primers(two inner primers and two outer primers)are designed for LAMP amplification and the conditions of the amplification are optimized，and then results show that gcnomic DNA of gosling plague virus detection sensitivity is 1 copy．The detection of the viruses of being separated and be preserved by the laboratory from clinic shows a good specificity of this method．The results of this study show that detection of gosling plague virus using LAMP is high sensitivity and specificity,rapid，easily to be operated and low-cost．So it is expected to develop into an effective and rapid detection of gosling plague virus infection，in addition to the clinical detection of value that the assay call quickly and accurately detect the gosling plague virus，referring tO clinical reference and reducing economic losses． Key words：gosling plague virus，LAMP,clinical application 扬州人学硕}：学位论文 4 缩写 APG K婚 BIP ELlSA FIP GPV LAMP mln PBS PCR rpm 英文名称 adenosine triphosphate 符号说明 Gosling plague virus loop—mediated isothermal amplification minute phosphate buffered saline polymerase chain reaction 中文名称 琼脂扩散试验 三磷酸腺苷 下游内引物 酶联免疫吸附试验 上游内引物 小鹅瘟病毒 环介导恒温扩增法 分钟 磷酸盐缓冲液 聚合酶链式反应 转／分钟 崔洪平t小鹅皇1．^MP检捌方法的建立和应用咀厘无锅地区小鹅毫流行状况调鸯 33 扬州大学学位论文原创性声明和版权使用授权书 学位论文原刨性声明 奉人声明：所呈交的学位论文是在导师指导下独立进行研究工作所取得的研究成果。除文 中已经标明引用的内容外．本论文不包含其他个人或集体已经发表的研究成果。对本文的研究 做出贡献的个人和集体．均已在文中以明确方式标明。本声明的法律结果由本人承担。 学位论文作者签名罐!‰jf 签字日期：—丫 年卜月叶Et 本人完全了解学校有关保留、使用学位论文的规定。即：学校有权保留并向国家有关部门