脐血间充质干细胞移植对脑梗死大鼠的影响及作用机制.docVIP

脐血间充质干细胞移植对脑梗死大鼠的影响及作用机制 　　[摘要] 目的 探讨脐血间充质干细胞（mesnchymal stem cells，MSCs）移植对脑梗死大鼠的影响及作用机制。 方法 体外培养脐血间充质干细胞并采用流式细胞仪检测细胞免疫表型，利用线栓法制作大鼠脑梗死模型；将120只大鼠随机分成MSCs移植组、对照组；对大鼠进行神经功能评价；Western-blot检测GFAP蛋白的表达。荧光显微镜观察大鼠海马结构变化及MSCs在大鼠脑内的分布。 结果 神经功能检测MSCs移植组神经功能评分降低，对照组神经功能评分升高，差异有统计学意义（P0.05）。MSCs移植组GFAP的表达量升高，7 d达高峰，对照组无明显变化（P0.05）。MSCs移植组MSCs在大鼠脑内分布良好，海马结构层次清晰，对照组海马神经组织结构紊乱，神经突触及细胞器溶解破坏。 结论 MSCs可促进大鼠脑梗死后神经功能恢复及海马结构的修复，增强神经可塑性。 　　[关键词] 脐血间充质干细胞；脑梗死；神经可塑性 　　[中图分类号] R743.33 [文献标识码] A [文章编号] 1673-9701（2015）14-0021-03 　　[Abstract] Objective To explore the effects and mechanism of implantation of umbilical blood mesenchymal stem cells （MSCs） on rats with cerebral infarction. Methods Umbilical blood MSCs were cultured in vivo， flow cytometry was applied to test cytometric immunophenotype， and model of rat cerebral infarction was made by suture method； 120 rats were randomly assigned to MSCs implantation group and control group； rats neurological function was evaluated； western-blot was applied to test the expression of GFAP protein. Fluorescence microscope was applied to observe changes of hippocampal formation in rats as well as the distribution of MSCs in rats brain. Results According to the test of neurological function， the score of neurological function in the experiment group was reduced， and the score in the control group was improved. The difference was statistically significant （P0.05）. Expression of GFAP in MSCs implantation group was increased with a peak in the 7th day， while no significant changes in the control group （P0.05）. According to the observation of immunofluorescence microscopy， the distribution of MSCs in rats brain was good in MSCs implantation group， and the hippocampal formation showed clear layers in the experiment group. Hippocampal structures in the control group were chaotic， and synapses and organelles were dissolved and compromised. Conclusion MSCs are able to promote the repair of hippocampal structures after cerebral infarction in rats， stren