过氧化物酶体增殖体激活受体γ激动剂对炎症状态下人肾小球系膜细胞ABCA1表达的影响.docVIP

过氧化物酶体增殖体激活受体γ激动剂对炎症状态下人肾小球系膜细胞ABCA1表达的影响 　　[摘要] 目的 研究过氧化物酶体增殖体激活受体γ（PPAR-γ）激动剂15-脱氧前列腺素J2（15d-PGJ2）对炎症状态下人肾小球系膜细胞（HMC）ATP结合盒转运体A1（ABCA1）表达的影响，探讨影响受体表达和延缓肾小球疾病进展的可能干预措施。 方法 培养的HMC随机分为5组继续培养24 h：空白组：普通培养的细胞；对照组：5 ng/mL白细胞介素-1β（IL-1β）；实验组：5 ng/mL IL-1β+2.5 μmol/L 15d-PGJ2，5 ng/mL IL-1β+5 μmol/L 15d-PGJ2，5 ng/mL IL-1β+10 μmol/L 15d-PGJ2，应用实时定量PCR法测定不同剂量PPAR-γ激动剂15d-PGJ2对IL-1β作用下HMC ABCA1 mRNA的影响。培养的HMC随机分为4组继续培养24 h：空白组：普通培养的细胞；对照组：5 ng/mL IL-1β；实验组：5 ng/mL IL-1β+5 μmol/L 15d-PGJ2，5 ng/mL IL-1β+10 μmol/L 15d-PGJ2，应用Western印迹方法测定不同剂量15d-PGJ2对IL-1β诱导的ABCA1蛋白表达的影响。 结果 炎症因子IL-1β能够抑制HMC的ABCA1表达，15d-PGJ2呈剂量依赖性地增加IL-1β抑制的ABCA1 mRNA和蛋白表达。与对照组（5 ng/mL IL-1β）比较，2.5、5、10 μmol/L 15d-PGJ2实验组ABCA1 mRNA表达分别升高1.34、2.15、2.88倍，5、10 μmol/L 15d-PGJ2实验组ABCA1蛋白表达分别升高1.16、1.54倍。 结论 PPAR-γ激动剂15d-PGJ2剂量依赖性改善IL-1β对ABCA1 mRNA和蛋白表达的抑制，对炎症导致的系膜细胞脂质失衡具有保护作用。 　　[关键词] 过氧化物酶体增殖体激活受体γ激动剂；系膜细胞；白细胞介素1β；ATP结合盒转运体A1 　　[中图分类号] R692 [文献标识码] A [文章编号] 1673-7210（2015）07（b）-0007-05 　　[Abstract] Objective To observe the protective effect of peroxisome proliferator activated receptor-γ （PPAR-γ） agonist for human mesangial cell （HMC） by the rgulation of ATP-binding cassette transporter A1 （ABCA1） when stimulated by interleukin-1β （IL-1β） from progression of kidney diseases. Methods HMC cultured for 24 hours were randomly divided into 5 groups： blank group： normal cultured cells； control group： cultured cells stimulated by dose of 5 ng/mL IL-1β； study group： cultured cells stimulated separately by 5 ng/mL IL-1β+2.5 μmol/L 15d-PGJ2， 5 ng/mL IL-1β+5 μmol/L 15d-PGJ2， 5 ng/mL IL-β+10 μmol/L 15d-PGJ2， the mRNA levels of ABCA1 were examined using Real-time PCR. Similarly， HMC cultured for 24 hours were randomly divided into 4 groups： blank group： normal cultured cells； control group： cultured cells stimulated by dose of 5 ng/mL IL-1β； study group： cultured cells stimulated separately by dose of 5 ng/mL IL-1β+5 μmol/L 15d-PGJ2， 5 ng/mL IL-β+10 μmol/L 15d-PGJ2， the protein levels of ABCA1 were detected by Western blot.