微孔板法与传统方法对比测定花菇、猴头菇和茶树菇的抗氧化活性.docVIP

微孔板法与传统方法对比测定花菇、猴头菇和茶树菇的抗氧化活性 [摘要] 目的 对比微孔板法和传统方法分光光度法测定花菇、猴头菇和茶树菇体外抗氧化活性的差异。 方法 超声波法提取3种菇的水提物和醇提物，以VC为对照品，均与底物1，1-二苯基-2-三硝基苯肼自由基（DPPH?）反应，其中微孔板法为微孔板结合酶标仪于517 nm波长处快速测定菇类提取物的DPPH?抑制率，传统分光光度法于紫外-可见分光光度计517 nm波长处检测。对2种方法的测定结果进行曲线拟合，并进行方法重复性对比。 结果 2种方法抗氧化结果IC50基本一致，曲线拟合度较好，差异无统计学意义（P 0.05）；其中对照品VC的微孔板法拟合模型公式为y 23.872Lnx+152.526，分光光度法模型公式为y 22.469Lnx+146.044，2种方法的模型中系数和常数经t检验差异有统计学意义（P 0.01），提示重叠率高。分别对3种样品的提取液进行曲线拟合，结果显示3种菇提取液2种抗氧化活性测定结果的曲线拟合度均较好，差异有统计学意义（P 0.05）。实验中微孔板法试剂用量较小，精密度更高。 结论 2种菇类体外抗氧化活性测定方法实验结果一致，差异不明显，微孔板法在试剂用量和精密度方面略具优势。 [关键词] 抗氧化活性；微孔板法；分光光度法 [中图分类号] R965 [文献标识码] A [文章编号] 1674-4721（2015）06（a）-0007-05 Comparison on the determination results of microplate method and traditional spectrophotometric method on antioxidant activity of Flower Mushroom，Hericium and Agrocybe Aegerita WANG Ming-juan1 WU Yan-shu2 LI Zhu3 WANG Lin-lin3▲ WANG Chen-lin3 1.Department of Pharmacy，the Third Affiliated Hospital of Henan University of Traditional Chinese Medicine，Zhengzhou 450008，China；2.Department of Pharmacy，the Second Hospital of Yulin City，Shaanxi Province，Yulin 719000，China；3.Pharmacy College，Henan University of Traditional Chinese Medicine，Zhengzhou 450046，China [Abstract] Objective To compare the determination results about microplate method and traditional spectrophotometric method on the antioxidant activity in vitro of Flower Mushroom，Hericium and Agrocybe Aegerita. Methods Ultrasonic extraction was used to extract the aqueous extracts and ethanol extracts of 3 mushrooms，VC was used as the reference substance，and given the reaction with the substrate 1，1-diphenyl-2-picrylhydrazyl radical 2，2-diphenyl-1-（2，4，6-trinitrophenyl） hydrazyl free radical （DPPH?）.Microplate method was that using microplate combined with microplate reader in 517 nm wavelength for determination of DPPH? inhibition ratio of mushroom extracts fastly，traditional spectrophotometric method was that determining in the UV Vis spectrophotometry in 517 nm wavelength for determination.Then the curve fi