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与酚类共色对花青素之稳定性及抗氧化力之影响
與酚類共色對花青素之穩定性及抗氧化力之影響
Effect of copigmentation with phenolic compounds on the
stability and antioxidant activity anthocyanins
研究生:黃愉婷 Yu-Ting Huang
指導教授:蔡碧仁 Pijen Tasi
【摘要】
花青素為自然界植物中色彩鮮豔的色素之一,維生素 C 是果汁中普遍存在的營養成分,但花青素在維生素C存在下,容易產生無色化合物而失去原有色澤,使其原本鮮豔的紅色褪色。為維持花青素顏色的穩定,本研究以洛神葵為材料,於維生素C 500 ppm 下 pH 2與pH 3模 pH值、濃度就維生素C、二氧化硫及溫度探討形成共維生素C的維生素C結果酚酸吸收波右移,;在增色效應方面,以效果最強,,最大吸光值的情形pH 3者增色的效果較pH 2好,波長位移則pH 3與pH 2解指數 DI值 進一步分析花青素率發Anthocyanins are one of the bright pigments in nature; vitamin C is a common nutritional composition in juices. Howerever, when anthocyanin and vitamin C coexist, it usually leads to the formation of colorless product and degradation of anthocyanin. In order to keep the color stability of anthocyanin, Roselle was targeted in this research. The objective was aimed to study the optimum condition pH and molar ratio for the copigment formation through five kinds of phenolic compounds ferulic acid, gallic acid, catechin, chlorogenic acid, and caffeic acid addition in two model systems pH 2 and pH 3 with 500 ppm vitamin C. A model system was used to investigate the change of color quality, patterns of anthocyanin, retention percentage of anthocyanin and vitamin C, and antioxidant capacity. In addition, the heating treatments, vitamin C and SO2 stability of the copigment, were investigated. It is anticipated that the copigmentation between anthocyanin and phenolic compounds may delay the anthocyanin degradation and improve its stability when coexisted with vitamin C.
Result showed that all the phenolic compounds favor the copigment formation, in both hyperchromic effect and bathochromic shift. Ferulic acid showed the greatest effect on bathochromic shift, followed by chlorogenic acid. Catechin and caffeic acid are strongest on hyperchromic effect. Both hyperchromic
effect and bathochromic shift increased with increasing molar ratio of phenolic compounds present. As in copigmentation effect at different pH values; in hyperchromic effect, the result of p
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