川崎病血清MMP—3、MMP—9及其TIMP—1水平变化及与冠状动脉病变的关系.docVIP

川崎病血清MMP—3、MMP—9及其TIMP—1水平变化及与冠状动脉病变的关系 　　[摘要] 目的 检测川崎病患儿血清中基质金属蛋白酶-3（MMP-3）、基质金属蛋白酶-9（MMP-9）及其特异性组织抑制剂-1（TIMP-1）水平的变化，以探讨上述因子水平的变化与冠状动脉病变的关系。 方法 收集2011年1月～2012年9月在本院儿科住院的川崎病患儿，设为观察组，采取ELISA法测定川崎病患儿急性期和恢复期血清MMP-3、MMP-9和TIMP-1的含量，并与同期进行健康体检的正常健康儿童（设为对照组）进行对比观察，通过心脏彩超检查明确川崎病患儿冠状动脉损伤情况。 结果 观察组与对照组MMP-3、MMP-9、TIMP-1检测值及MMP-3/TIMP-1、MMP-9/TIMP-1比较，差异均有统计学意义（P 0.05、P 0.01）；观察组中有冠状动脉病变组各检测因子结果与无冠状动脉病变组比较差异有统计学意义（P 0.01）。 结论 MMP-3、MMP-9与TIMP-1均参与了川崎病的病理生理过程，对早期诊断冠状动脉病变有提示作用，且MMP-3/TIMP-1、MMP-9/TIMP-1比值持续失调可判断冠状动脉炎症病变的严重程度。 　　[关键词] 川崎病；基质金属蛋白酶-3；基质金属蛋白酶-9；特异性组织抑制剂-1；冠状动脉病变；酶联免疫吸附法 　　[中图分类号] R725.4 [文献标识码] A [文章编号] 1674-4721（2013）07（a）-0008-03 　　The relationship of Kawasaki disease and serum MMP-3，MMP-9 and TIMP-1 levels and its with lesion of coronary artery 　　ZHANG Zhi-ling 　　Department of Pediatrics，Dalang Hospital of Dongguan City in Guangdong Province，Dongguan 523770，China 　　[Abstract] Objective To detect the change of serum matrix metalloproteinases in children with Kawasaki disease，matrix metalloproteinases-3（MMP-3），-9 （MMP-9） and its specific tissue inhibitors-1 （TIMP-1） levels，to research the relation on change of the factor levels and coronary artery lesions. Methods The inpatient children with Kawasaki disease in Department of Pediatrics of our hospital from January 2011 to September 2012 were collected as observation group，the MMP-3，MMP-9 and TIMP-1 contents in the serum of children with Kawasaki disease were determined by ELISA method in acute period and convalescent period，and compared with those of normal healthy children （as the control group） at the same time，the coronary artery lesions of the children with Kawasaki disease were determined by echocardiography. Results The MMP-3，MMP-9，TIMP-1，MMP-9/TIMP-1 and MMP-3/TIMP-1 detection value were compared in the observation group and control group，there were statistically significant differences （P 0.05，P 0.01）；The MMP-3，MMP-9，TIMP-1，MMP-9/TIMP-1 and MMP-3/TIMP-1 detection value in the observation g