金钠多对体外缺氧致人大动脉内皮细胞分泌内皮素的影响.docVIP

金钠多对体外缺氧致人大动脉内皮细胞分泌内皮素的影响 作者：李鸣皋，马贵喜，韩磊，刘玉，李靖，蒙果，刘昕 【关键词】 ；内皮缩血管肽类 Effects of Ginaton on hypoxiainduced endothelin secretion from human aortic endothelial cells 　　【Abstract】　AIM: To explore the effects of Ginaton at different concentrations on the hypoxiainduced endothelin (ET) secretion from cultured vascular endothelial cells (VECs). METHODS: Cultured VECs were divided into 5 groups: control group, simple hypoxia group, highconcentration Ginaton group, moderateconcentration Ginaton group, and lowconcentration Ginaton group. For hypoxia, all groups except control group were put in a hypobaric chamber at the height of 5000 m for 30 min. The ET contents in supernatant of VECs before hypoxia and 0.5 h after hypoxia, 6 h after hypoxia as well as 24 h after hypoxia were measured with radioimmunoassay. RESULTS: ① Simple hypoxia promoted VECs secretion of ET and ET content increased significantly at 0.5 h after hypoxia and reached the peak at 6 h (P＜0.01). ② Ginaton inhibited VECs secretion of ET induced by hypoxia. At the time of 0.5 h after hypoxia, highconcentration Ginaton was more effective than moderateconcentration and lowconcentration Ginaton (P＜0.01). At the time of 6 h and 24 h after hypoxia, moderateconcentration Ginaton was more significantly effective than highconcentration and lowconcentration Ginaton (P＜0.05). CONCLUSION: Ginaton has significant protective effects on hypoxiainduced ET secretion from cultured VECs and the effects of moderateconcentration Ginaton are more obvious compared with those of highconcentration and lowconcentration Ginaton. 　　【Keywords】 endothelial, vascular/cytology; endothelins; ginaton; anoxia 　　【摘要】 目的： 探讨不同浓度金钠多对由缺氧所致培养的血管内皮细胞分泌内皮素功能的影响. 方法： 将培养的血管内皮细胞分为空白对照组、单纯缺氧组、缺氧加高浓度金钠多组、缺氧加中浓度金钠多组和缺氧加低浓度金钠多组. 采用低压舱上升至5000 m高度、停留30 min，对培养的血管内皮细胞进行缺氧. 用放射免疫法测定缺氧前和缺氧后0.5, 6, 24 h各组细胞培养液中内皮素含量. 结果： ① 急性缺氧可显著促进血管内皮细胞分泌内皮素，缺氧后0.5 h内皮素含量即显著升高，于缺氧后6h分泌内皮素含量最高. ② 金钠多能明显抑制缺氧促血管内皮细胞分泌内皮素的作用，在缺氧后0.5 h表现