不同培养条件对铁皮石斛类原球茎生物反应器培养的影响.PDFVIP

不同培养条件对铁皮石斛类原球茎生物反应器培养的影响 徐步青 1, 2，李振中 2，张 俊 2，王 芬 1，刘幸佳 1，崔永一 3* 1. 浙江农林大学园林学院，浙江 临安 311300 2. 浙江农林大学林学基础省级实验教学示范中心，浙江 临安 311300 3. 浙江农林大学农业与食品科学学院，浙江 临安 311300 摘 要：目的 研究不同培养条件对铁皮石斛类原球茎在生物反应器中培养的影响。方法 采用接种量、通气量、蔗糖浓度、 光照强度等单因素试验设计，烘干法测定生物量，苯酚-硫酸法测定多糖量，数据采用 SPSS16.0 软件分析。结果 在生物反 应器培养条件下，利于铁皮石斛类原球茎增殖和多糖积累的培养基组分为：1/2 MS 基本培养基添加 1.0 mg/L NAA、5%椰乳、 3%蔗糖，pH 值为 6.0。接种量为 40 g/L，采用孔径为 15 μm 的多孔喷头，通气量用 1.0 L/min 和 0. 5 L/min 交替使用以及光 照强度为 2 000 lx 等培养条件有效地提高类原球茎增殖系数和多糖量。结论 不同的培养条件对铁皮石斛类原球茎的生长、 多糖的变化有显著影响，适宜的培养条件对铁皮石斛类原球茎的生物量和主要药用成分的生产具有重要的实践意义。 关键词：铁皮石斛；类原球茎；培养条件；生物反应器；增殖系数；多糖 中图分类号：R282.21 文献标志码：A 文章编号：0253 - 2670(2014)05 - 0 - 05 DOI: 10.7501/j.issn.0253-2670.2014.05. Effect of different culture conditions on protocorm-like bodies of Dendrobium candidum in bioreactor culture XU Bu-qing1, 2, lI Zhen-zhong2, ZHANG Jun2, WANG Fen1, LIU Xing-jia1, CUI Yong-yi3 1. School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Lin’an 311300, China 2. Basic Experiment Teaching Center of Forestry; Zhejiang Agriculture and Forestry University, Lin’an 311300, Chian 3. School of Agriculture and Food Science, Zhejiang Agriculture and Forestry University, Lin’an 311300, China Abstract: Objective To explore the effect of different culture conditions on protocorm-like bodies (PLBs ) of Dendrobium candidum in bioreactor culture. Methods Inoculation quantity, ventilation, sucrose concentration, and light intensity were taken to carriy out the single factor experiment design. Biomass was determined by drying method, polysaccharide content by phenol-sulfuric acid method and SPSS16.0 software was used for data analysis. Results Under the bioreactor culture, the nutrient media components for the proliferation of PLBs of D. candidum and accumulation of polysaccharide were1/2 MS medium supplemented with 1.0 mg/L NAA, 5% coconutmilk, and 3% sucrose, 6.0 pH, was benefit for the proliferation and polysaccharide accumulation of the PLBs