非洲马瘟病毒VP7基因拼接、表达及重组ELISA方法的建立与初步应用.pdfVIP

畜牧兽医学报??，???：??—?? ?? Veterinaria ? Zootechnica ??? ????????????VP7???????????????? ????ELISA???????????????????? 高志强，张鹤晓’，赖平安，谷强，蒲静，汪琳，乔彩霞，吴丹，柏贬铎，张伟 ?本┏鋈刖乘笱榧煲呔侄?锼笠咧行模?本???? ??????????????????????????????????????????(AHSV)??????????????????????????VP7???????????????? 隆予????陆ㄖ刈赫鹇磒?????，将??????4緽????，筑?? mmol??L??诱导，努嚣基 ??以包涵体的形式获褥离效表达。通过?????约癊??试验证明表达产物具有良努的反应原性。以纯化 ??????????????????????????????????????????AHSV??????????ELISA???????????????????????????????? ????0??25pg??mL????????????????????1t ?，待检血清阳性临界值初步定为????么朔椒ê蜕唐坊疎??试 ??????????184?????????????????????????? ??????????????????????????????????lsA 中国分类号：??．???：??．??? ????????????A文章编曙：???????????—? Development and Application ? the Recombinant ELISA Based ? the ????? and ????? ? VP7 ?? ? African ???? Virus GA0 ?????琙?? ?????琇? Ping-an??GUQiang??PU??， ?? Lin??QIA0Cai-xia??WU??珺? Ya-duo??ZHANGWei (Animal ????? ??? ? ??ing Entry??Exit????? and ????? ???，＆?蒳? ???，??? Abstract??ADNA fragment??whichencodes the ?? ??? ???? ? VP7 ? African ???? ???????? assembled ??????，?? ??? ?? pET-30a??Targetprotein expressed ? ?? ?? level ? inclusion ?? after the recombinant ????猇? plasmid was transformed in- ? BL21(D??3)??and