多核巨细胞在小鼠黑色素瘤细胞转移中的作用机制研究.pdfVIP

中华临床医师杂志(电子版)2013 年 7 月第 7 卷第 14 期 Chin J Clinicians(Electronic Edition),July 15,2013,Vol.7,No.14 ·6457· ·基础论著· 多核巨细胞在小鼠黑色素瘤细胞转移中的 作用机制研究 米蕊芳 刘福生 金贵善 【摘要】 目的 研究多核巨细胞在黑色素瘤转移中的作用及其相应分子机制。方法 用聚乙二醇细胞 融合法体外获取黑色素瘤多核巨细胞，通过流式分选黑色素瘤多核巨细胞。小鼠皮下及尾静脉注射黑色素 瘤多核巨细胞并用游标卡尺测量皮下肿瘤体积，电子天平称重荷瘤肺组织以定量肿瘤转移能力。Affymatrix 基因芯片分析差异表达的基因，Ingenuity Pathways Analysis（IPA）软件进行基因功能分析。结果 细胞融 合并流式分选成功获得黑色素瘤多核巨细胞。黑色素瘤多核巨细胞皮下生长速度减慢但肺转移能力明显增 强。黑色素瘤多核巨细胞与转移相关的 β-Tubulin 组基因表达明显升高。黑色素瘤多核巨细胞的染色体呈现 稳定性。结论 黑色素瘤多核巨细胞促进黑色素瘤细胞转移能力，黑色素瘤多核巨细胞与转移相关的 β-Tubulin 组基因表达升高。 【关键词】 巨细胞； 黑色素瘤； 肿瘤转移； 小鼠 Melanoma multinucleated giant cells acquired enhanced metastasis potential MI Rui-fang, LIU Fu-sheng, JIN Gui-shan. Brain Tumor Research Center, Beijing Neurosurgical Institute, Beijing Tiantan Hospital Affiliated to Capital Medical University, Beijing 100050, China Corresponding author: JIN Gui-shan,Email:guishanjin7@ 【Abstract】 Objective To study the role of melanoma multinucleated giant cells in tumor metastasis and the corresponding molecular mechanism. Methods Melanoma multinucleated giant cells were obtained through the polyethylene glycol(PEG)fusion method following fluorescence activated cell sorting (FACS). To detect the tumor growth rate in vivo, subcutaneous tumor volumes were measured using a vernier caliper. To analyze the tumor metastasis potential, cells were injected intravenously, and the collected lung metastasis samples were weighed by an electronic balance. Differentially expressed genes between the melanoma multinucleated giant cells and parental cells were detected by the Affymatrix gene chip following the gene function analysis by Ingenuity Pathways Analysis(IPA). Results Melanoma multinucleated giant cells were successfully obtained through PEG cell fusion method following FACS. The metastasis potential of melanoma multinucleated giant cells was significantly enhanced, in contrast to their subcutaneously decreased growth rate. A group of β-Tubulin gene related to meta