Amphotericin B and lecithin - cholesterol liposomes degradation kinetics.docVIP

 PAGE \* MERGEFORMAT 15 Amphotericin B and lecithin - cholesterol liposomes degradation kinetics Authors: Xiao Ping Yang Li, Yu Shi Jinsong Zeng Qun Rainbow [Abstract] Objective To investigate the in lecithin, cholesterol membranes prepared amphotericin B liposome stability and degradation kinetics. Methods of light and heat on high-performance liquid chromatography study of amphotericin B solution and the liposomes drug stability and degradation kinetics and degradation kinetics of fitting the model, seeking degradation half-life balance. The results of amphotericin B liposome solution and its photodegradation, respectively, and in line with a zero order kinetics, the degradation half-lives were 2.6 and 24.7d; thermal degradation process were consistent with zero order kinetics and Higuchi equation; 40 ℃ under the half-life were 27.8 and 1532.8d; 60 ℃ under the half-lives were 8.2 and 17.9d. Conclusions can be significantly reduced liposome amphotericin B, light and heat degradation, degradation half-life extending drugs, amphotericin B significantly increased stability. [Keywords:] amphotericin B; lecithin; cholesterol; liposomes; stability; degradation kinetics ABSTRACT Objective To study the stability and degradation kinetics of amphotericin B in liposome. Methods The light and thermal stabilities of amphotericin B in liposome and solution were determined by detecting its content using HPLC and the degradation kinetics was also investigated. Results The light degradation kinetics of amphotericin B in liposome and solution fit the zero-and first-order equations, and their half lives were 24.7d and 2.7d, respectively. While their thermal degradation kinetics complied with the Higuchi model and zero-order equations, respectively. The amphotericin B in liposome was more stable than that in solution at 40 ℃ and 60 ℃ . The half lives of amphotericin B in liposome and solution were 1,532.8 d and 27.8d at 40 ℃ , but 17.9d and 8.2d at 60 ℃ , respectively. Con