Amphotericin B Determination of iron content.docVIP

 PAGE \* MERGEFORMAT 7 Amphotericin B Determination of iron content Author: Du Xiaoxia Wu Zhong-Tao Song Jian Meng, such as Zhang Jian [Abstract] Objective To determine the amphotericin B in the iron content. Methods of amphotericin B completely ash ash dissolved in dilute hydrochloric acid obtained in solution Fe (III) under acid conditions with potassium thiocyanate to form a red soluble thiocyanate ligand iron ions, using colorimetric determination of iron content in . Results complex at a wavelength of 500nm at the maximum absorption, Fe (III) concentration of 0.1 ~ 2.0μ g/ml to obey Beer’s law within the framework of the standard curve regression equation: Y = 0.1766X 0.0159, correlation coefficient r = 0.9995, coefficient of variation 1.23 %, recovery rate 98% ~ 103%. Conclusion In this study, iron Pharmacopoeia Limit test method and colorimetric method with the establishment of a quantitative test for iron, the method is simple, rapid, accurate and satisfactory results. [Keywords:] amphotericin B; ash; iron; thiocyanate method; colorimetry; determination Amphotericin B from the culture medium of Streptomyces isolated from polyene macrolide antifungal agent for systemic fungal infections of the preferred drug. Of amphotericin B is less stable raw material medicine, studies have shown that a high iron content is the main factor affecting its stability, so the iron content of amphotericin B control is an important indicator of impurity control. Pharmacopoeia Thiocyanate method is only available for a limited examination of iron, are unsure of its content [1]. In this study, combination of thiocyanate method and colorimetric method can be accurately measured amphotericin B in the iron content, good reproducibility of this Act, with satisfactory results. 1 Materials and methods 1.1 Materials and Instruments Fe (III) stock solution (0.1mg/ml), said an accurate take 0.4317g NH4Fe (SO4) 2.12 H2O (AR) set 500ml flask, add water, after being dissolved in su