Depression on Dingzhixiaowan on the structure of rat hippocampus.docVIP

 PAGE \* MERGEFORMAT 10 Depression on Dingzhixiaowan on the structure of rat hippocampus Of: wake up to the Chairui Hua Shan Yan Tak Hung [Abstract]: Objective To study the morphology around the hippocampus dingzhixiaowan antidepressant mechanisms. Methods 40 female rats were divided into control group, depression group (model group), distilled water group (DW group) and dingzhixiaowan group (CMB), Open field behavior scores were detected, ELISA assay of serum cortisol and estradiol (E2) levels, HE staining and electron microscope morphology of neurons in hippocampal CA3, immunohistochemical detection of dentate gyrus stem cells (NSC) proliferation. Results Compared with control group, model group and group behavior DW score and serum E2 levels were significantly decreased cortisol levels and significantly reduced NSC proliferation of hippocampal area CA3 neurons obvious behavioral medicine group scores and no significant change in serum E2 levels, cortisol significantly reduced, NSC proliferation and a significant increase in hippocampal CA3 neurons is normal. Conclusion dingzhixiaowan to protect the hippocampus, which may be one of the mechanisms of its anti-depressant. [Keywords:] estradiol depression dingzhixiaowan cortisol rat neural stem cells Abstract: Objective To study the anti-depression mechanism of Dingzhixiaowan concerning on histology of hippocampal formation. Methods 40 female rats were divided into control, depression model group (model group), distilled water group (DW group) and Dingzhixiaowan group (Chinese herb group ). Behavior points were measured by open field method. Serum concentrations of cortisol and estradiol (E2) were checked with ELISA. HE staining and electron microscopy were applied to observe the histology of neurons in hippocampal formation. Immunohistochemistry method was employed to detect Nestin expression . Results Compared with the control, behavior points, serum E2 level and Nestin expression decreased obvio