Determination of total saponins Prunella.docVIP

 PAGE \* MERGEFORMAT 8 Determination of total saponins Prunella [Abstract] [Objective] The determination of Prunella total saponins in Radix. [Methods] Prunella sample solution extracted with methanol, 5% vanillin - glacial acetic acid, color, Determination of total saponin content in the samples. [Results] saponins in the range of 26.95 ~ 94.31g linear relationship between absorbance recovery was 97.0% ~ 100.5%, and three batches of samples Prunella total saponins of 2.02% ~ 2.56%. [ Conclusion] Determination of total saponins in P. vulgaris, the method is accurate and reliable, easy to operate. [Keywords:] Prunella; total saponins; spectrophotometry of content; Abstract: [Objective] To measure the content of total saponins in selfheal herb. [Method] Extract selfheal resolution with carbinol ultrasound, 5% vanilla aldehyde-icy vinegar acid coloring, measure content of total saponins in sample with spectrophotometry. [Result] In 26.95 ~ 94.31g, the total saponin has good linear relationship with absorbency, the recycle rate is 97.0% ~ 100.5%; 3 batches of selfheal samples have saponins 2.02% ~ 2.56%. [Conclusion] Spectrophotometry measuring saponins in selfheal is correct, reliable and easy of operation. Keywords:: selfheal; total saponins; spectrophotometry; content measure Labiatae plant Prunella Prunella (Brunellae cum fructu L. dry ear, a clear fire, eyesight, relieves congestion, swelling of the effect, for the red eyes and throat, headache, dizziness, M. scrofulaceum, milk carbuncle pain, hypertension, acute jaundice, hepatitis and other infectious diseases [1]. Prunella contain oleanolic acid and other triterpenoids, -sitosterol and other steroid components, luteolin and other flavonoids, coumarins , phenylpropanoids, organic acids, volatile oil constituents, pharmacological studies have shown, which has a blood pressure, hypoglycemic, anti-inflammatory, immunosuppressive, anti-bacterial, anti-virus and so on [2]. this summer by ultravio