Determination of two kinds of detectors in Dangshen Content of atractylenoide Ⅲ.docVIP

 PAGE \* MERGEFORMAT 9 Determination of two kinds of detectors in Dangshen Content of atractylenoide Ⅲ [Keywords:] detector Abstract: Objective: To Evaporative Light Scattering Detector (ELSD) Determination of Codonopsis atractylenoide Ⅲ content, and with diode array detector (PAD) determination results were compared. METHODS: RP-HPLC method, Hypersil ODS2 column (5 μ m, 4.6 mm * 250 mm), methanol - water as mobile phase (67:33), flow rate 1.0 mL / min, column temperature was 30 ℃ . Results: ELSD Detection Codonopsis Radix atractylenolide Ⅲ regression equation is A = 1.30 * 104C-2.33 * 104, the correlation coefficient r = 0.9938, average recovery was 103.65%, RSD 0.93%. Conclusion: The ELSD measured Codonopsis Radix herb to the content within the lipid Ⅲ PAD measured in Radix Codonopsis Ⅲ lipid content of the herb in basically the same, to ELSDRPHPLC Determination of the herb Radix Codonopsis Ⅲ within the lipid content of the The method is simple, easy, accurate and reliable. Keywords:: evaporative light scattering detector; RPHPLC; diode array detector; atractylenolide Ⅲ ; Determination Codonopsis is commonly used Chinese herbal medicine, there is qi, beneficial blood, Sheng Jin functions, also have elevated blood sugar, promote hematopoietic function, regulation and anti-ulcer and other gastrointestinal contraction in multiple roles. ‘The People’s Republic of China Pharmacopoeia ‘2000 edition reproduces Codonopsis Codonopsis Pilosula (Franch.) Nannf., Su-Hua Codonopsis Codonopsis Pilosula Nannf.var.modesta (Nannf.) LTshen or River Codonopsis Codonopsis tangshen Oliv. Three kinds of 〔 1 〕 . WANG Zheng-Tao and other isolated from Codonopsis within sesquiterpene esters atractylenolide Ⅲ been proved to have significant anti-inflammatory activity〕 〔 2-3, and with Hao Guiming, respectively, using forward and reverse high performance liquid chromatography Analysis of the Codonopsis Radix herb to the content within the lipid 〔Ⅲ〕 4-5, the detectors are use