EDTA-K2-induced platelet aggregation effects on the blood cell count.docVIP

 PAGE \* MERGEFORMAT 5 EDTA-K2-induced platelet aggregation effects on the blood cell count [Abstract] Objective To study the EDTA-K2-induced platelet aggregation caused by the blood analyzer counting RBC, WBC and PLT values change. Methods The blood analyzer anticoagulant EDTA-K2, while hand-counting specimens and smear microscopy, and compared to the corresponding conclusion. Results of blood analyzer part of the EDTA-K2 anti-clotting platelet count lower than the results of manual counting method, while the results of white blood cell count was higher than the latter, the difference between the two have clinical significance. Conclusion For the EDTA-K2 anticoagulant for individual patients with blood platelet aggregation may occur, thus leading to reduction of platelet pseudo PTCP (pseudothrombocytopenia), WBC count increased false, so they should pay attention to, be promptly corrected, to avoid unnecessary checks and treatment. [Keywords:] EDTA-K2 platelet aggregation in blood count Hematology analyzer has become popular in various hospitals across the country, compared with the traditional manual method for the advantage is simple, fast, repeatable, but many external factors may affect the accuracy of detection. EDTA-K2 anti-clotting caused by individual human platelet aggregation occurs is a typical example, it can lead to EDTA-dependent pseudo-thrombocytopenia (EDTA-dependent PTCP) [1], but also caused the number of white blood cell count high, causing the clinical misdiagnosis, this experiment of seven cases of platelet aggregation in patients analyzed. 1 Data and methods 1.1 Detection of the object in August 2007 ~ August 2008 EDTA-K2 anticoagulant with platelet aggregation in 7 patients, including 3 males and 4 females; aged 15 to 55 years old with an average of 34 years. 1.2 Instrument and reagents Shenzhen Mindray BC-3200 hematology analyzer reagents and equipment, quality control product is PHC-3D blood cell quality control material (Bio-Tec