Effects of resveratrol on guinea pig ventricular myocytes L.docVIP

 PAGE \* MERGEFORMAT 7 Effects of resveratrol on guinea pig ventricular myocytes L Author: Yi Fang Fang, TANG Qi-chu, Feng Si-ting, Wang Teng [Keywords:] resveratrol; whole-cell patch-clamp; calcium currents Effects of resveratrol on Ltype calcium current in isolated ventricular myocytes of guinea pig [Abstract] AIM: To investigate the effects of resveratrol on the Ltype calcium current (ICaL) in isolated ventricular myocytes of guinea pig. METHODS: Single ventricular cell was obtained from healthy guinea pig heart using enzymatic dissociation methods. The wholecell patch clamp was used to record the ICaL following the administration of resveratrol at 1, 50, 100 μ mol / L. RESULTS: ① Resveratrol reduced the ICaL in a concentrationdependent manner, but it had no effect on IV shape of ICaL. ② Resveratrol markedly shifted the steadystate activation curve of ICaL to the right, and V1 / 2 value increased from (-18.97 ± 4.08) mV in control to (-15.97 ± 4.13) mV in the presence of resveratrol (n = 7, P = 0.02), the κ value from ( 3.64 ± 0.56) to (3.82 ± 0.60) (n = 7, P = 0.67). ③ Inactivation curve of ICaL was not affected significantly. ④ The time constant of reactivation of ICaL was reduced from (98.50 ± 9.00) ms to (54.53 ± 4.27) ms (n = 6, P = 0.000) by resveratrol. CONCLUSION: Resveratrol inhibited the Ltype calcium channel, mainly acting on the activity and recovery processes. [Keywords] resveratrol; wholecell patch clamp; calcium current [Abstract] Objective: To study the effect of resveratrol on normal guinea pig ventricular myocytes L-type calcium current (ICaL). Methods: Using whole-cell patch-clamp technique to record before and after different concentrations of resveratrol on the ICaL impact. Results: ① resveratrol in a concentration-dependent block ICaL, but the activation potential, peak potential, reversal potential were not affected; ② resveratrol ICaL steady-state activation curve shifted to the right to make, V1 / 2 by contrast, (-