Effects of resveratrol on the expression of colorectal cancer and its hTERT promoter of.docVIP

 PAGE \* MERGEFORMAT 13 Effects of resveratrol on the expression of colorectal cancer and its hTERT promoter of Of: Wang Xiaoyan, Fan Yu, Zhang particular calendar, Zhong Ximing [Abstract] Objective: To observe the effect of resveratrol on the proliferation of colon cancer cells, and from the human telomerase reverse transcriptase (human telomerase reverse transcriptase, hTERT) expression and promoter areas of possible mechanism. Methods : Treatment with different concentrations of resveratrol LS 174T colon cancer cells, the cells were collected, respectively, by fluorescent real-time quantitative RT PCR and Western blot cancer hTERT mRNA and protein levels. liposome transfection method used will carry hTERT promoter and reporter gene plasmid Tai LS 174T colon cancer cells after different concentrations of resveratrol, 48 h incubation, the detection of firefly luciferase reporter gene activity. Results: Resveratrol treatment of colorectal cancer cells, proliferation was inhibited in a dose related. carcinoma hTERT mRNA and protein levels were significantly reduced in a dose and time dependent. resveratrol treated group firefly luciferase activity decreased in a dose dependent manner. Conclusion: hTERT hTERT promoter activity and decrease the expression of resveratrol inhibition of cancer cell proliferation may be an important mechanism. [Keywords:] resveratrol; colorectal cancer; human telomerase reverse transcriptase; promoter [Abstract] Objective: To investigate the effect of resveratrol on human telomerase reverse transcriptase (hTERT) and its promoter of human colorectal cancer cell. Methods: After LS 174T human colorectal cancer cell were treated with different dose of resveratrol, mRNA and protein of hTERT were determined by real time PCR and Western blot, respectively. The plasmid inserted hTERT promoter was transected into LS 174T cells by oligofecamine 2000, and different doses of resveratrol were added into culture media 2 hours later. Aft