Erythromycin on peripheral blood mononuclear cells in asthmatic rats phosphorylation of JNK expression.docVIP

 PAGE \* MERGEFORMAT 15 Erythromycin on peripheral blood mononuclear cells in asthmatic rats phosphorylation of JNK expression Of: Wucheng Yun, Zhang recent wave, Li Zhiqiang, Dai Yuanrong [Abstract] Objective: To investigate the peripheral blood mononuclear cells in asthmatic rats (PBMCs) JNK phosphorylation of their expression changes and erythromycin. METHODS: The isolated and cultured normal control group, asthma group, rats treated with erythromycin PBMCs. were detected by ELISA in supernatant IL-4, IL-5 concentration, reverse transcription polymerase chain reaction (RT-PCR), Western Bloting detected PBMCs JNKmRNA, the expression of phosphorylated JNK. Results: The asthma group PBMCs JNK protein phosphorylation, mRNA expression and cell supernatant levels of IL-4, IL-5 levels than normal control group was significantly higher (P lt;0.05), erythromycin treatment JNKmRNA, protein expression and phosphorylation Cell supernatant IL-4, IL-5 levels were significantly lower than that of asthma (P lt;0.05). by linear correlation analysis, PBMCs JNK protein phosphorylation and cell supernatant IL-4, IL-5 in expression showed a significant positive correlation between (r = 0.74 and 0.66, P all lt;0.05). Conclusions: JNK may be involved in the pathogenesis of bronchial asthma. erythromycin may be that by acting on target to play the anti-JNK inflammatory effect. [Keywords:] bronchial asthma; peripheral blood mononuclear cells; JNK protein kinase; erythromycin Abstract: Objective: To study the changes of the expression of JNK mitogen-activated protein kinase (JNK-MAPK) in peripheral blood mononuclear cells (PBMCs) in rats with asthma and the effects of erythromycin on it. Methods: PBMCs were collected and cultured from rats with asthma, normal subjects and erythromycin-treated group. The levels of protein of IL-4, IL-5 in plasma, JNKmRNA and protein of JNK in PBMCs were detected with enzyme linked immunosorbent assay (ELISA), reverse transcription polymera