Esophageal visceral sensitivity to the establishment and evaluation of animal models.docVIP

 PAGE \* MERGEFORMAT 12 Esophageal visceral sensitivity to the establishment and evaluation of animal models [Abstract] Objective To establish the animal model of esophageal visceral hypersensitivity. Methods intraperitoneal injection of chicken ovalbumin (OVA) sensitized the basis of a joint approach to esophageal acid perfusion in SD rats, using immunohistochemical methods and micro-image analysis evaluation of visceral hypersensitivity - a rat model of esophageal chemical stimuli reliability. The basis of the results of OVA sensitized rats co-esophageal acid perfusion was activated a complex and extensive brain network, in frontoparietal cortex, insula, cingulate cortex, central amygdaloid nucleus, K  lliker-Fuse nucleus, nucleus ambiguus, parabrachial nucleus, hypothalamic paraventricular nucleus, hypothalamic paraventricular nucleus, the trigeminal next to the nucleus, solitary tract nucleus, area postrema, medullary reticular nucleus and other Fos-like immunoreactivity (FLI) neurons were significantly higher than the number of the other groups ( P amp;lt;0.05). Model group in the central amygdala, parabrachial nucleus, paraventricular nucleus, the trigeminal next to the nucleus, solitary tract nucleus of the FLI-positive product of the average optical density (OD) values were significantly higher than the other groups (P amp;lt;0.05). Conclusion Pre-intraperitoneal injection of chicken egg white protein-based allergens combined esophageal acid perfusion can be successfully established animal model of esophageal visceral hypersensitivity. [Keywords:] visceral hypersensitivity; c-fos; Immunohistochemistry; esophageal acid infusion; egg white protein [Fund] National Natural Science Foundation of China (30,700,362) Establish and evaluation of the rat model of esophageal visceral hypersensitivity Abstract: Objective To establish a reliable rat model of esophageal visceral hypersensitivity. Methods Healthy Sprague-Dawley (SD) rats received basal oval