FACS 17estradiol on H2O2-induced retinal cell apoptosis induced.docVIP

 PAGE \* MERGEFORMAT 14 FACS 17estradiol on H2O2-induced retinal cell apoptosis induced Of: Jia Guorong, Yu Xiaorui, Bo Li, WANG Shu, Han Yan [Abstract] the purpose of using flow cytometry (FACS) detection of H2O2-induced apoptosis of retinal nerve cells, and 17 estradiol (E2) protective effects on cells. Methods of newborn SD rats within 24h of retinal nerve cells in the original culture, cell culture to be 4-5d, after treatment by different factors, cell viability by MTT assay, apoptosis by FACS detection rate. Results 200mol / L of H2O2 could effectively induce apoptosis in the retina, low concentrations The H2O2 can induce apoptosis, but the high concentration of H2O2 induced apoptosis in normal cells also leads to sharp drop in rates, 10mol / L of E2 effective against H2O2-induced apoptosis of retinal neurons, low concentrations of E2 the cells did not show a protective effect, high concentrations of E2 cells but showed toxic effects. Conclusion E2 a certain concentration can be within a certain range against H2O2-induced apoptosis of retinal neurons, showing the protective effect on neurons. [Keywords:] 17 estradiol, H2O2, the retina, apoptosis, neuroprotection ABSTRACT: Objective To observe H2O2 induced neuronal cell apoptosis and neuroprotection of 17E2 by the technique of FACS. Methods The primary retina neuronal cells of neonatal SD rats which were born in 24h were cultured. After 4-5 days, we treated the cells using different factors, then observed the cell viability by MTT method and the cell apoptosis by FACS method, respectively. Results H2O2 treatment (200mol / L) could effectively induce the cultured primary retina neuron cell apoptosis. The low concentration of H2O2 could not affect the cells, but the high concentration treatment could induce cell apoptosis, at the same time leading to normal cell decrease sharply; E2 treatment (10mol / L) showed neuroprotection on the primary cultured retina neuron cells, but the low concentration of