Fake blood coagulation caused by difficulties in 2 cases with.docVIP

 PAGE \* MERGEFORMAT 7 Fake blood coagulation caused by difficulties in 2 cases with [Keywords:] Blood Transfusion; serum; blood typing and cross matching of blood A case report Case 1: male patients, aged 77, for “gallstones” in September 2008 admitted for surgery. Preoperative examination: body temperature: 37.2 , pulse: 86 times / min, BP: 120/80mmHg, Hb 89g / L, RBC 2.2 1012 / L, WBC 3.0 109 / L, PLT 93 109 / L, blood type: O type RhD (+), cardiopulmonary and renal function was normal. no history of blood transfusion. preoperative preparation of blood, the cross-matching of blood micro- the main side column gel agglutination and censorship. Case 2: male patients, aged 78, admitted to hospital for treatment of cerebral infarction and anemia. Had 5 blood transfusions, hospital examination: mental outlook of the poor, pale appearance was anemia, blood pressure 90/60mmHg, Hb 71g / L, RBC 1.8 1012 / L, WBC 2.1 109 / L, PLT 71 109 / L, blood type: O type RhD (+). prepare for a transfusion, cross matching the primary side of the micro-column gel agglutination and censorship. 2 blood group serology 2.1 The anti-A reagent and method of anti-B, anti-D from the Changchun Biological Technology Co., Boyd Company, Ltd., batch number are:and 1745080. IgG anti-human globulin reagent polybrene (Polybrene) antibody screening reagents and cells were blood from the Shanghai biomedical limited liability company, respectively,20070515lot. All tests are using the classical tube test. ABO blood group testing positive and negative stereotypes of conventional methods, RhD blood group with the saline method, antibody screening and cross-matching of blood were used saline media law, anti-human globulin test, three technical methods polybrene test operation [1]. 2.2 Identification of patients with blood type O-blood are RhD (+) 2.3 in the blood serum antibody screening results in Table 1. Table 1 antibody screening results of