Flavonoids in the spring and HPLC Bupleurum fingerprint.docVIP

 PAGE \* MERGEFORMAT 13 Flavonoids in the spring and HPLC Bupleurum fingerprint Of: Li Jing, Wang Bo, Zhang Lei, Panlu Lin, Zhao, Zhen Ouyang [Abstract] Objective: To establish a spring Bupleurum analysis of total flavonoids and flavonoid content of High Performance Liquid Chromatography fingerprint. Methods: visible spectrophotometry of total flavonoids content, using high performance liquid chromatography with different harvest Bupleurum different parts of flavonoids in the spring to analyze the fingerprint to establish the spring Bupleurum. Results: April to May harvest of spring Bupleurum higher flavonoid content, total flavonoid content of leafgt; whole plantgt; stemgt; roots. application “similarity of chromatographic fingerprint evaluation software” generated a total of spring herbs Bupleurum diagram, a total of 12 peaks, separation between the peaks better, precision, repeatability and stability test of the Total peak relative retention time and relative peak area RSD was less than 3%, in line with fingerprint requirements. CONCLUSION: The harvest of the various medicinal parts of flavonoids spring Bupleurum significant difference, the study of resources for the spring Bupleurum the comprehensive utilization of development to provide a scientific basis. [Key Words] high performance liquid chromatography, fingerprinting, total flavonoids, Spring Bupleurum [Abstract] Objective: To establish the method of total flavonoids and HPLC fingerprint in Bupleurum scorzonerifolium Willd .. Methods: The content of flavonoids was determined with ultraviolet visible spectrophotometry, The HPLC was used to study of chromatogram of different collection period and parts, The fingerprint of Bupleurum scorzonerifolium Willd. was established. Results: The better harvest time of Bupleurum scorzonerifolium Willd. was from April to May and flavonoids content was that: leafgt; whole plantgt; stemgt; root. The standard fingerprint of Bupleurum scorzonerifolium Willd.