FMDV Asiaa recombinant protein epitopes and immune activity.docVIP

 PAGE \* MERGEFORMAT 12 FMDV Asiaa recombinant protein epitopes and immune activity Of: Zhang Fei Cao Weihong Zhao ZHANG Yong-sheng Xu Haifei by Hu Xiaoping Wan Min Yu Yongli Wang Liying Wang Abstract Objective To construct that can prevent foot and mouth disease virus type Asia (FMDV) infection epitope recombinant protein vaccine and its immunological research. Methods Asia type FMDV VP1 epitopes on B cells and T cell epitopes of amino acid sequences of antigenic sites, design sites that contain the above, with the best conformation of the recombinant protein, named “CZ 1”. Through methods such as PCR and gene cloning and its expression plasmid was constructed in E. coli BL21 (DE3) induced expression. By nickel-affinity chromatography and G 25 gel filtration purification and refolding, obtained with immunological activity of the recombinant protein CZ 1. Obtained with immune guinea pig model for FMDV Asia and antibodies in the serum, and through the cells and test and test in suckling mice, guinea pig serum in the detection of the protective anti-FMDV neutralizing antibody titers. The results of the recombinant protein was able to induce in vivo anti-Asia type FDMV a protective neutralizing antibodies. Conclusion The recombinant protein is expected to develop into infection prevention Asia type FDMV new vaccines. Keywords: genetic engineering vaccine; FDMV; recombinant protein; activity was detected Foot and mouth disease (FMD) by the FMD virus (FMDV) were caused by humans and animals suffering from an acute febrile highly contagious infectious disease 1. Conventional FMD vaccine attenuated vaccine or inactivated vaccine, though immunogenic good, but there is not completely inactivated, and inadequate protection, that may cause risk of transmission of the virus from spreading, the insecurity spurred the search for new vaccines. Studies have shown, T and B cell antigen in series connection is built in situ genetic engineering vaccine