Focal cerebral ischemia in type 2 diabetes iNOS expression in hippocampus and the intervention of procyanidins.docVIP

 PAGE \* MERGEFORMAT 12 Focal cerebral ischemia in type 2 diabetes iNOS expression in hippocampus and the intervention of procyanidins [Abstract] Objective focal cerebral ischemia in type 2 diabetes in the rat brain induced by nitric oxide synthase (iNOS content changes and proanthocyanidins (procyanidin, PC on its expression. Methods rats were randomly assigned 30 patients divided into sham operation group, ischemia alone type 2 diabetes low dose group and PC group (50 mg / kg, medium dose group (100 mg / kg and high dose group (200 mg / kg, 24 h after ischemia the HE staining, immunohistochemical determination of iNOS content in the hippocampus. focal ischemia in type 2 diabetic rats, iNOS activity decreased significantly in hippocampus (P lt;0.01), PC low, medium and high dose group were reduced to varying degrees iNOS activity (P lt;0.01), to improve the extent of the damage of nerve cells: one for middle and high dose group was significantly (P lt;0.05), but the middle and high dose groups were not significantly different (Pgt; 0.05). Conclusions PC-2 Diabetes can reduce focal cerebral ischemia in rats iNOS activity in the hippocampus. [Keywords:] diabetes, cerebral ischemia induced by procyanidins nitric oxide synthase Abstract: Objective To investigate the content of induced nitricoxide synthase in hippocampus tissue of type 2 diabetes mellitus rats with focal cerebral ischemia and effects of procyanidin (PC) on it. Methods Thirty SD rats were divided into sham-operation, model, low-dose PC, mid-dose PC and high-dose PC group. After ischemia for 24h, we performed hematoxylin and eosin (HE) staining; the expression of iNOS was examined by immunohistochemical SABC method. Results Compared with sham-operation group, the increased number of the positive iNOS cells in cerebral hippocampus of model group, the difference between the two groups was of significant (P lt;0.01). Compared with model group, the PC groups possessed lower the positive cells of