Forkhead proteins p66shc and longevity.docVIP

 PAGE \* MERGEFORMAT 3 Forkhead proteins p66shc and longevity [Abstract] p66shc is a proto-oncogene shc encoded protein, p66shc knockout increased by H2O2 and UV-induced apoptosis resistance. P66shc-/ - mice could increase paraquat resistance and extended life span. FKHRL1 and DAF16 is a fork head protein transcription factor family members, and appropriately increase the transcriptional activity of fork head protein, extend life, and it can inhibit the phosphorylation of its transcriptional activity. Oxidative stress can lead to a concentration-dependent FKHRL1 phosphorylation. p66shc-/ - cells in the H2O2 treated FKHRL1 phosphorylation was no significant change, indicating that oxidative stress-modified fork head protein in the regulation of the phosphorylation of the need p66shc participation. [Keywords:] fork head protein p66shc DAF16 Longevity Longevity has been a dream of mankind, though found in a number of factors can shorten life expectancy, but so far, people still could not find the availability of extended life span approach. However, in recent years, genetic studies found that knockout of certain genes can extend the life of C. elegans or mouse, which gives longevity research brings new hope. 1 p66shc and longevity Mammalian proto-oncogene shc encoding three proteins of similar molecular weight: 52K (P52shc), 46K (p46shc) and 66K (p66shc). They all have src homology domain (SH2), a collagen - homology region (CH1) and the phosphotyrosine-binding domain (PTB). P66shc only contains amino-terminal region (CH2) (Figure 1). Figure 1 p66shc, p52shc, and p46shcde structure is illustrated S: S36, P66shc the major serine phosphorylation sites; Y: tyrosine phosphorylation site To study the stress response in p66shc’s role, Migliaccio et al [1] using the measured p66shc phosphorylation-sensitive phospho-qmino-acid analysis of the studies have shown that UV and H2O2-treated cells with post-phosphoserine significant and sustained increas