HPLC determination of berberine hydrochloride in skin and the content of pills.docVIP

 PAGE \* MERGEFORMAT 9 HPLC determination of berberine hydrochloride in skin and the content of pills Of: Luo Yifen, Chengyan Yang, Xie Youlian, Huang Xi Yue, Chen Xinyu [Abstract] Objective To develop a skin pill determination of berberine hydrochloride. Methods using HPLC, the column for the Welch Malerials XB C18 column, acetonitrile and 0.02 mol L-1 phosphoric acid (25:75) as mobile phase The flow rate was 1.0 mL min-1, the detection wavelength was 424 nm, column temperature was 35 . the results of the concentration of berberine hydrochloride 3.814 ~ 38.14 g mL-1 range of good linear relationship between peak area; average recovery was 99.1%, RSD was 0.8% (n = 9); The detection limit was 0.942 7 ng. Conclusion The method is simple, accurate and can effectively control the quality of the preparation. [Keywords:] Skin Pill; Berberine; high performance liquid chromatography Abstract: Objective To establish an HPLC method for determining the content of berberine hydrochloride in Pifu pills. Methods A Welch Materials XB C18 column was used to analyze the sample by using acetonitrile 0.02 mol / L phosphoric acid solution (25:75) as mobile phase . The flow rate was 1.0 mL / min and detection wavelength was 424 nm. Results The standard curve for berberine hydrochloride was linear in the concentration range of 3.814 ~ 38.14 g / mL. The average recovery was 99.1% with RSD 0.8% (n = 9). Conclusion This method was simple, accurate and reliable for the quality control of Pifu pills. Keywords:: Pifu pills; berberine hydrochloride; HPLC Treats skin pill by the Commissioner, Cnidium, wind etc., with nourishing and expelling wind, dampness itching effect. In order to better control the quality of the preparation, through access to prescription medicines in plant analysis of the information, choose prescription medicines off the main Cork [1] Determination of berberine hydrochloride to study. in the light of the literature [1,2], based on the established