HPLC determination of cytidine triphosphate disodium in the concentration.docVIP

 PAGE \* MERGEFORMAT 6 HPLC determination of cytidine triphosphate disodium in the concentration Author: Qiu-Xiang Wang, Ji magnificent, Zhang Yantong [Abstract] Objective: To establish a cytidine triphosphate disodium injection determination methods. Methods: A high performance liquid chromatography (HPLC). Results: This method allows Cytidine Disodium Triphosphate and Cytidine diphosphate, disodium cytidine monophosphate disodium be a very good separation. Conclusion: The method applicable to the preparation of quality control. [Keywords:] cytidine triphosphate disodium; high-performance liquid chromatography; RP-column Cytidine triphosphate disodium salt (CTP) is a nucleoside analogue drugs in the body involved in the synthesis of nucleic acid and phospholipid metabolism, promoting protein synthesis, mainly used to treat a variety of clinical causes of nervous system diseases and vascular sclerosis . CTP is very unstable, are heat-sensitive drugs, which lower the product is disodium cytidine diphosphate (CDP) with a cytidine disodium phosphate (CMP). Previous determination errors larger, and the operation cumbersome. In this paper, a dedicated high-performance liquid chromatography determination of CTP concentration column, and with electrophoresis [1] as control, with rapid, sensitive and other characteristics, and can degrade the product of their qualitative and quantitative analysis, are presented below. An apparatus and reagents CLASS-VPLC-10A liquid chromatograph, Shimadzu Corporation; C18 -4: Water column: Dogsil5μ m4.6 * 25mm, Dalian Berry Group chromatography Center, CMP, CDP, CTP reference substance, Hangzhou, the United States and Asia Bio-Technology Co., Ltd.Provide . 2 Methods and Results 2.1 Chromatographic conditions Dogsil C 18-45μ m4.6 * 250mm column; detection wavelength 280nm; column temperature 35 ℃ ; a mobile phase of 0.2mol.L; phosphate buffer - methanol (90:10); flow 1ml.min. 2.2 The reference substance for the test it