HPLC determination of glycyrrhizin and glycyrrhizic acid content of the study.docVIP

 PAGE \* MERGEFORMAT 16 HPLC determination of glycyrrhizin and glycyrrhizic acid content of the study 【Abstract】 Objective To establish glycyrrhizin and glycyrrhizic acid HPLC assay method. Method uses KromasiL-C18 (4.6 mm × 200 mm, 5 μm) column, glycyrrhizin using acetonitrile -0.5% acetic acid (20:80) as mobile phase, detection wavelength 276 nm, column temperature was room temperature; glycyrrhizic acid mobile phase, of methanol -0.2 mol * L-1 ammonium acetate solution - glacial acetic acid (67:32:1), detection wavelength 252 nm, column temperature at room temperature. Both flow rate were 1.0 ml * min-1, using external standard were determined. The results of glycyrrhizin in the 2.7 ~ 27 μg * ml-1 showed good linear relationship within the framework of (r = 0.999 7); glycyrrhizic acid in the 6 ~ 36 μg * ml-1 showed good linear relationship within the framework of (r = 0.999 8) ; glycyrrhizin and glycyrrhizic acid, the average recoveries were 101.4% and 101.3%, the RSD values were 1.18% and 1.22%. Conclusion The method is simple, accurate and a good linear correlation coefficient, and the stability and good repeatability for crude extracts of licorice flavonoids and purified in glycyrrhizin and glycyrrhizic acid content determination. Keywords: high-performance liquid chromatography; glycyrrhizin; glycyrrhizic acid Abstract: ObjectiveTo establish method for determination of liquiritin and glycyrrhizic acid by HPLC.MethodsKromasil-C18 column (4.6 mm × 200 mm, 5 μm) was used, HPLC analysis for liquiritin was performed with room temperature and the mobile phase consisted of acetonitrile-0.5 % glacial acetic acid (20:80), the detection wavelength was at 276 nm; HPLC analysis glycyrrhizic acid was performed with room temperature and the mobile phase consisted of methanol-0.2 mol * L-1 ammonium acetate-glacial acetic acid (67: 32:1), the detection wavelength was at 252 nm. The flow rate was 1.0 ml * min-1. ResultsThe linear range of liquiritin