HPLC Determination of Ningxia produced four kinds of content Bupleurum kaerophyllin.docVIP

 PAGE \* MERGEFORMAT 5 HPLC Determination of Ningxia produced four kinds of content Bupleurum kaerophyllin Abstract HPLC Determination of Ningxia produced four kinds of Bupleurum ingredient in lignans (kaerophyllin) content of less advanced sample extraction with methanol. The chromatographic conditions were: ODS-C18 column (150mm * 6mm), mobile phase was methanol - water (70:30), detection wavelength 328nm. The results show that production in Ningxia var.parvifolium Bupleurum, Bupleurum short stem, hollow Bupleurum, Bupleurum in the Qinling contain kaerophyllin. Keywords: HPLC method; kaerophyllin; Ningxia production Bupleurum China has more than 20 species of Bupleurum species for medicinal, Ningxia, there are 10 kinds of 〔 1〕 contain saikosaponin ad. Var.parvifolium have a higher content of Bupleurum lignan ingredients, is different from a distinctive feature of Bupleurum 〔 2〕 . To systematically examine the quality of Bupleurum, the author produced four kinds of Bupleurum in Ningxia had Kaerophyllin determination. An instrument and reagent 1.1 Instrument LC-6A high performance liquid chromatography (Japan). 1.2 reagent Kaerophyllin (Toho University, Japan, from the pharmacy section, after UV, IR, MS, 1H-NMR, 13C-NMR identification); Bupleurum 〔 collected in Guyuan County, Ningxia for Drug Control, Xing Rui Zhuren pharmacists identified as: Bupleurum (B.chinense DC), lobular black Bupleurum (B.smithii wolff var.parvifolium Shan et Y. Li), a short stem Bupleurum (B.pusillum Krylov), hollow Bupleurum (B.longicaule var.franchelii Boiss), Qinling Bupleurum (B.longicaule var.giraldii wolff)〕 . 2 Methods and Results 2.1 Chromatographic conditions Column Shimpack CLC-ODS-C18 (150mm * 6mm), mobile phase: methanol - water (70:30), flow rate 1ml/min, sample volume 15μ l, detection wavelength 328nm. 2.2 The standard curve Precision Weigh Kaerophyllin 5mg, constant volume with methanol, dissolved in 10ml Liang Ping, the standard solution is made; precision dra