HPLC Determination of paeoniflorin back pain tablets.docVIP

 PAGE \* MERGEFORMAT 8 HPLC Determination of paeoniflorin back pain tablets [Abstract] Objective To develop low back pain, the film method for the determination of paeoniflorin. Methods using high performance liquid chromatography, Shimpack VP-ODS C18 (4.6 mm * 150 mm, 5 μ m) as column, methanol -0.05% phosphoric acid (volume ratio 40:65) as mobile phase, detection wavelength was 230 nm, flow rate To 1.0 ml / min. The results of paeoniflorin linear range 0.1448 ~ 2.8960 μ g, the average recovery was 100.4%, RSD of 1.53% (n = 6). Conclusion The method is simple, accurate, high sensitivity, good reproducibility and can effectively control the quality of low back pain tablets. [Keywords:] low back pain tablets; paeoniflorin; high-performance liquid chromatography Low back pain tablets containing relevant to ‘ministerial standard’ first five volumes of Chinese medicine, from Eucommia leaf, Dipsacus, TPG and other 12 Chinese herbal medicines, has a strong waist kidney, promoting blood circulation analgesic effect, good clinical results [1]. Have not received ministerial standards set out in the assay of the preparation of indicators. Literature has been used in the preparation of HPLC determination of psoralen and psoralen content [2,3]. TPG has Qingreliangxue, Sanyu analgesic effect, to reflect the effect of low back pain, analgesic tablets blood circulation, so this choice of paeoniflorin measured as indicators of low back pain tablets using HPLC determination of paeoniflorin in preparation [4], in order to the formulation of the preparation of quality standards to provide experimental basis. An instrument and reagent Agilent 1100 High Performance Liquid Chromatography (USA, Agilent); Branson SB3200 ultrasonic cleaning instrument (Shanghai, Branson); column was Shimpack VP-ODS C18 (4.6 mm * 150 mm, 5 μ m). Low back pain tablets (Guilin Sanjin Pharmaceutical Co., Ltd., substrate weight 0.35 g); paeoniflorin reference substance (for determination by the Ch