HPLC determination of vitamin K1 in the drug content of freeze-dried milk.docVIP

 PAGE \* MERGEFORMAT 5 HPLC determination of vitamin K1 in the drug content of freeze-dried milk [Abstract] Objective: To establish a RP-HPLC determination of vitamin K1 in the drug content of freeze-dried milk. Methods: The freeze-dried with isopropyl alcohol after breast reconstruction demulsification. Using Hypersil BDS C18 column (4.6 mm * 250 mm, 5 μ m), with methanol: ethanol (70:30) as mobile phase, detection wavelength of 254 nm, flow rate of 1.0 mL / min, injection volume 20 μ L, column temperature was 35 ℃ . Results: The preparation of auxiliary interference-free determination of the main drugs, vitamin K1 in the 25 ~ 250 mg / L within the linear relationship between peak area and concentration of a good average recovery was 100.99%, RSD 0.24%. Conclusion: isopropoxide demulsification good effect, and no effect on the determination of the main drugs. This method is simple, rapid, accurate, and can be used for the preparation of the quality control. [Keywords:] high-performance liquid chromatography; vitamin K1; freeze-dried milk; Determination [Abstract] Objective: To determine the content of Vitamin K1 in lyophilized emulsion by reversed  phase high performance liquid chromatography.Methods: The reconstituted Vitamin K1 emulsion was mixed with isopropyl alcohol. A Hypersil BDS C18 column (4.6 mm * 250 mm, 5 μ m) was used. The mobile phase was methanol  dehydrated alcohol (70:30), the UV detector at wavelength 254 nm and the flow rate 1.0 mL / min. The injection volume was 20 μ L and the column temperature 35 ℃ . Results: The excipients did not interfere with the determination of Vitamin K1. The method proved to be linear within the range of 25 ~ 250 mg / L. The recovery of the assay was 100.99% and RSD was 0.24%. Conclusions: Isopropyl alcohol can play a good role in emulsion breaking, meanwhile have no impact on measurement of Vitamin K1. The method appears to be simple, speedy, accurate and can be used to control the preparation quality. [K