Human lung squamous cell carcinoma and inflammatory pseudo-tumor protein expression profile differences in the initial establishment of.docVIP

 PAGE \* MERGEFORMAT 13 Human lung squamous cell carcinoma and inflammatory pseudo-tumor protein expression profile differences in the initial establishment of Author: Li Cui, Chao-Jun Duan, Zhi-Qiang Xiao, Yi-Hong, Tang ginseng E, Chen-Chu [Abstract] Objective To establish a high resolution and repeatability better human lung squamous cell carcinoma and inflammatory pseudotumor of the organization’s two-dimensional gel electrophoresis patterns, and identify the differential expression of identified proteins. The use of solid-phase pH gradient two-dimensional gel electrophoresis separation of human lung squamous cell carcinoma, and inflammatory pseudotumor of the organization’s total protein, gels by silver staining, the use of ImageMaster 2D graphics software for comparative analysis to identify differentially expressed proteins, matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI  TOF  MS) and database search identified some differences in protein spots. Results (1) has been higher resolution, better repeatability of human lung squamous cell carcinoma, and inflammatory pseudotumor of the organization’s two-dimensional gel electrophoresis map of the same cancer tissue, and inflammatory pseudotumor of the organization were carried out three times two-dimensional gel electrophoresis, three protein gel, the average points were 1569 ± 78 and 1487 ± 64, respectively, the average match point 1454 ± 37 and 1370 ± 43, matching rate of 92.7% and 92.1%; the same cancer The three-point position in the protein gel has good repeatability between the different gel protein spots in the IEF direction of deviation (0.733 ± 0.101) mm, in the SDS  PAGE direction of the deviation (0.925 ± 0.207) mm. (2) a comparative analysis of lung squamous cell carcinoma, and inflammatory pseudotumor of the organization’s two-dimensional gel electrophoresis map, with an average match points for the 1142 ± 51, of 23 different protein spots of the pepti