hTERT gene promoter regulation of HSV.docVIP

 PAGE \* MERGEFORMAT 8 hTERT gene promoter regulation of HSV Author: Bian card, ZHANG Hui-Zhong, Ren Ji-hong, ZHAO Hui, Cheng Shi-Yin [Keywords:] telomere, terminal transferase; promoter region (genetics); cervical cancer; tk gene [Abstract] AIM: To observe the killing effect of HSVtk / GCV system on cervical carcinoma cells under the control of hTERT (human telomerase reverse transcriptase) core promoter. METHODS: An eukaryotic expression vector containing HSVtk gene under the control of hTERT core promoter was constructed and was transfected into cervical carcinoma cells (HeLa) and vessel endothelial cells (ECV304) by liposome method. Transfected cells were then selected with G418 and RTPCR was used to detect the tk gene expression in HeLa cells and ECV304 cells. After GCV was added , flow cytometry method were applied to investigate its cell killing effect. RESULTS: pCIneo / hTERTtk / GCV system under the control of hTERT induced the apoptosis in more than 36.7% of cervical carcinoma cells, but not in normal vessel endothelial cells. CONCLUSION: hTERT gene core promoter is tumorspecific and may be useful in tk gene therapy of cervical carcinoma and in reducing the side effects of the therapy. [Keywords] telomerase; promoter regions (genetics); cervix neoplasms; tk gene [Abstract] Objective: To study the human telomerase reverse transcriptase (hTERT) gene core promoter regulation of the human herpes simplex virus thymidine kinase gene / ganciclovir (HSVtk / GCV) system for cervical cancer cells in vitro. Methods: Construction of hTERT gene promoter regulation of the tk gene eukaryotic expression vector pCIneo / hTERTtk, respectively liposome transfected cervical cancer cells (HeLa) and normal vascular endothelial cells (ECV304), neomycin (G418) screened positive for clonal expansion. Comparison between two kinds of cells, using RTPCR expression of tk gene; to give the prodrug ganciclovir (GCV), with flow cytometry hTERT regulation of HSVtk / GCV system