Hydroxypropyl-β -cyclodextrin by capillary electrophoresis Determination of 1,2.docVIP

 PAGE \* MERGEFORMAT 11 Hydroxypropyl-β -cyclodextrin by capillary electrophoresis Determination of 1,2 Authors: Zhao, Xing-Bin Yang, JIANG Ru, SUN Xiao-Li, LIU Wen-Min, ZHANG Sheng-Yong [Keywords:] Hydroxypropyl Enantiomeric excess analysis of 1,2 diphenyl1, 2ethanediol by capillary electrophoresis using HPβ CD as chiral selectors [Abstract] AIM: To develop a new method to determine enantiomeric excesses (ee%) of 1,2 diphenyl1, 2ethanediol by capillary electrophoresis (CE). METHODS: The effects of the concentration of buffer and hydropropylβ cyclodextrin (HPβ CD), pH, running voltage and capillary temperature on the enantioseparation of 1,2 diphenyl1, 2ethanediol were investigated by CE. Enantiomeric excesses of 4 samples synthesized by asymmetric dihydroxylation were investigated and the results determined by CE method were then evaluated by comparison with those by HPLC. RESULTS: Under the optimized conditions of 200 mmol / L boric acid buffer of pH 9.8 containing 15 mmol / L HPβ CD, at capillary temperature 20 ℃ and separation voltage 15 kV, 1,2 diphenyl1, 2ethanediol was completely separated with resolution as high as 3.35. Synchronously, the ee% of the 4 batches of samples determined by CE was consistent with that by HPLC. CONCLUSION: This method is simple, precise and can be applied to the chiral separation of 1,2 diphenyl1, 2ethanediol enantiomers and determination of ee%. [Keywords] electrophoresis, capillary; 1,2 diphenyl1, 2ethanediol; enantiomer; chiral separation; stereoisomerism [Abstract] Objective: To establish a capillary electrophoresis (CE) determination of (E) 1,2 2 Asymmetric Dihydroxylation of styrene 1,2-diphenyl-ethylene glycol reaction product enantiomeric excess (ee%) value of the new method . Methods: HPβ CD as the chiral selector, using capillary zone electrophoresis method research background electrolyte concentration, pH, cyclodextrin concentration, separation voltage, temperature and other parameters on 1,2-diphenyl-glycol