Hypericum perforatum extract of Hypericum perforatum L. Study of Separation Process.docVIP

 PAGE \* MERGEFORMAT 10 Hypericum perforatum extract of Hypericum perforatum L. Study of Separation Process Author: Cheng Li Hui, Wang Zhuang, Li Xiao Meng 【Abstract】 Objective To investigate the Hypericum perforatum extract from the separation of hypericin process. Methods of ethanol extract from Hypericum perforatum Hypericum perforatum, and then S898 resin with silica gel column chromatography separation and purification of hypericin extracts. The results of the product in the gold content of prime pick up 2.17%. Conclusion The method is simple, low consumption, high levels of product hypericin. Keywords: Hypericum perforatum; extraction; separation; hypericin Of Hypericum perforatum Hypericum perforatumL. Yes Clusiaceae Hypericum (Hypericum) plant the whole plant of Hypericum perforatum. Qingrejiedu convergence of bleeding, dampness and other effects. Hypericin (Hypericin) is Hypericum perforatum the most biologically active substances. In recent years, studies have shown that hypericin with the anti-depressant, anti-bacterial anti-inflammatory, anti-DNA and RNA viruses, the promotion of memory and other pharmacological activity [1]. Hypericum perforatum in China is rich in resources, but because the whole plant of Hypericum perforatum contained in the Su-Jin-Tao of only a few extreme, it is only through chemical extraction and refining them in order to reach the international market of Hypericum perforatum extract of Hypericum pigment content is more than 0.3% of requirements. In this study, pairs of hypericin extraction, separation and purification process to explore in order to improve the content of Hypericum perforatum products. An instrument and reagent 1.1 Instrument HP8453UV-Vis absorption spectrometer (Hewlett-Packard), RE-52A rotary evaporators,, HHS21-N electric thermostat water bath pan. 1.2 Thin-layer chromatography silica gel reagent G (Qingdao Marine Chemical Co., Ltd.), eth