Mesenchymal stem cells in vivo tracing and Repair Experimental study of intervertebral disc degeneration.docVIP

 PAGE \* MERGEFORMAT 16 Mesenchymal stem cells in vivo tracing and Repair Experimental study of intervertebral disc degeneration Of: Cao Ye, Wu Xiaotao, Wang Yuntao, Yan Hui deep, Shao contribution [Abstract] Objective: To investigate the bone marrow mesenchymal stem cells (marrow mesenchymal stromal cells, MSCs) and in vivo tracing methods markers, and the feasibility of treating intervertebral disc degeneration. METHODS: Rabbit MSCs cultured in vitro, and purified in vitro amplification, using superparamagnetic iron oxide (superparamagnetic iron oxide, SPIO) magnetic particles were labeled with MTT assay 1,3,5,7 d cell activity. will be marked cells were implanted within the intervertebral disc degeneration. 2,4,6,8 weeks were used phloroglucinol Determination of changes in proteoglycan content, immunohistochemical determination of the change of type collagen content, with the MR scan of labeled stem cells in the intervertebral disc distribution of tracer . The data were analyzed statistically. Results: MTT assay showed that, SPIO of no effect on the biological activity of MSCs, MSCs can be effectively labeled SPIO. the use of MR scanning can be observed in the distribution of the body and found that stem cells taken place in the surrounding nucleus migration .8 weeks the experimental group and type collagen proteoglycan content than the model group, the difference was statistically significant. CONCLUSION: SPIO labeled MSCs can be effective in vivo tracking in vivo in parallel, without affecting cell viability, is the ideal tracer. SPIO labeled MSCs after transplantation can increase the nucleus in the content of the extracellular matrix, thus delaying the disc degeneration. [Keywords:] bone marrow mesenchymal stem cells; superparamagnetic iron oxide; magnetic resonance imaging; disc degeneration; rabbit Mesenchymal stem cells (marrow mesenchymal stromal cells, MSCs) has a strong self-renewal and differentiation capacity for a variety of mature