Non-methylated CpG-containing bifidobacteria DNA on mouse bone marrow cell maturation of dendritic.doc

 PAGE \* MERGEFORMAT 8 Non-methylated CpG-containing bifidobacteria DNA on mouse bone marrow cell maturation of dendritic Author: An Wei, Zhao Qing Miao, Li Yalin, Li Song, Li Kang, Song-gang [Keywords:] methylation Stimulatory effect of bifidobacteria DNA containing unmethylated CpG motifs on maturation of bone marrowderived dendritic cells [Abstract] AIM: To explore the effect of bifidobacteria DNA containing unmethylated CpG motifs (CpG DNA) on the maturation of bone marrowderived dendritic cells (BMDC). METHODS: The genomic DNA of bifidobacteria was extracted and purified, and the methylated degree of CpG motifs was tested. Cell phenotypes and antigens uptake of BMDC were analyzed by flow cytometry. The levels of IL6, IL12 (p40) and TNFα were detected by ELISA and T cell proliferation was detected by 3HTdR incorportation test. RESULTS: Treated with bifidobacteria DNA for 24 h, BMDC expressed a high level of Ia, CD40, CD86 and CD11c molecules, significantly increased the secretion of IL6, IL12 (p40) and TNFα and significantly enhanced the pro liferation of allogenic and homogenic T cells. CONCLUSION: Bifidobacteria DNA has remarkable stimulatory effects on BMDC maturation and antigenpresenting function. [Keywords] bifidobacteria DNA; dendritic cells; mice [Abstract] Objective: To explore the bifidobacteria DNA on mouse bone marrow dendritic cells (BMDC) mature. Methods: The purified extract of Bifidobacterium DNA, and identification of DNA products degree of methylation of CpG motifs; FACS detection of BMDC’s phagocytosis and surface marker molecules, ELISA assay BMDC secreted levels of cytokines, 3HTdR incorporation assay T lymphocyte proliferation. Results: Bifidobacterium DNA induced by 24 h after BMDC high expression Ia, CD40, CD86 and CD11c molecules, obviously increase in IL6, IL12 (p40), TNFα secretion, significantly enhance the allogeneic and autologous T cell proliferation capacity. Conclusion: Bifidobacterium DNA can promote BMDC matured