North Korea in Liaoning produced in different medicinal parts of the active ingredient of Epimedium Determination_0.docVIP

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North Korea in Liaoning produced in different medicinal parts of the active ingredient of Epimedium Determination_0.doc

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North Korea in Liaoning produced in different medicinal parts of the active ingredient of Epimedium Determination_0

North Korea in Liaoning produced in different medicinal parts of the active ingredient of Epimedium Determination Study: Single Na, Road King before, Wang Jing, Sun Wei, Yang [Abstract] Objective To determine the different areas of Liaoning in different medicinal parts of the Korean Epimedium icariin and total flavonoids. Icariin as an indicator method, using high performance liquid chromatography HPLC Epimedium , Determination of total flavonoids. the result leaves the content of icariin 0.197% ~ 0.347% in the range of icariin content in stem 0.043% ~ 0.050% in the range of leaf flavonoids content of 6.68% ~ 8.85% in the range of total flavonoids in the stem in 1.3% to 2.7% range. icariin in 0.3 ~ 1.8 μg (r = 0.999 9) and 0.5 ~ 3 μg / mL (r = 0.999 9) a good linear relationship. icariin, the average recovery was 99.43% (n = 6, RSD = 0.29%), total flavonoids was 100.38% (n = 9, RSD = 1.60%). Conclusion Liaoning Korean Epimedium different regions of different active ingredient content, different medicinal parts of the content of total flavonoids and icariin quite different leaves of total flavonoids and icariin much higher than the stem. [Keywords:] Korean epimedium total flavonoids, icariin, determination Abstract: Objective To determine the content of icariin and flavonids in Epimedium koreanum Nakai of different place of production and different pharmaceutical genetic in Liaoning Province. Methods Icariin was used as the chemical reference substance to establish HPLC and ultraviolet spectrophotometry methods for content determination of icariin and total flavonoids. Results The content of icariin in leaves was from 0.197% to 0.347%, and in stem was from 0.043% to 0.050%. The content of total flavonids in leaves was from 6.68% to 8.85%, and in stem was from 1.3 % to 2.7%. The calibration curves of icariin was in good linearity over the range of 0.3 ~ 1.8 μg (r = 0.999 9) and total flavonids was 0.5 ~ 3 μg / mL (r = 0.999 9). The average recovery of ic