On RP-HPLC method for determination of aristolochic acid content optimization.docVIP

 PAGE \* MERGEFORMAT 10 On RP-HPLC method for determination of aristolochic acid content optimization Of: Du Xiaoxi, Zhou Yuehua, Road King before, Han Na, Zhou Juan, BI Kai-shun [Abstract] Objective To optimize the performance liquid chromatography analysis of aristolochic acid content. Methods by comparing different extraction solvents and different extraction methods to determine a more reasonable method for the preparation of test solution. Comparison of different mobile phase conditions on the aristolochic Determination of acid content, choose the content of chromatographic conditions. measured at different wavelengths, the minimum detection limits of trace analysis to determine the conditions. The results of the test solution prepared with 70% methanol in direct heating reflux extraction efficiency than other extraction methods . to do when you can choose the general content of mobile phase was methanol -1% acetic acid solution (70:30) or acetonitrile -0.3% (NH4) 2CO3 (pH = 7.5) (25:75), 250 nm or 319 nm as detection wavelength . do trace analysis should choose mobile phase of acetonitrile -0.3% (NH4) 2CO3 (pH = 7.5) (25:75), and select the detection wavelength was 224 nm, the minimum detectable amount of up to 0.02 ng. Conclusions The laboratory The method can be accurate and reliable analysis of aristolochic acid content and for trace inspection. [Keywords:] aristolochic acid, HPLC, determination Abstract: Objective To optimize the RP-HPLC procedure for the determination of aristolochic acid. Method Different extraction solvents and methods have been screened to get the most efficient way for sample preparation. The influence of different mobile systems on quantitation were compared to choose an approprate mobile system for determination. Besides, the minimum limits of different detecting waves were measured to give the method to detect trace amount of aristolochic acid. Result Refluxing with 70% methanol was better than other ways in sample