Peripheral blood of children FAAS determination of five kinds of elements in.docVIP

 PAGE \* MERGEFORMAT 6 Peripheral blood of children FAAS determination of five kinds of elements in [Keywords:] of children in the five kinds of elements in peripheral blood At present, for the blood calcium, magnesium, copper, iron, zinc and five kinds of elements in the determination of the venous blood samples were widely used, and by flame atomic absorption spectrometry (FAAS) determination. As the collected blood can not easily be parents and children, for which use of auto-steady flow pulse sampling technique, both in the atomic absorption spectrometer is connected to an auto-steady flow into the injector pulse to micro-sampling method instead of the traditional continuous sampling method , Flame atomic absorption spectrometry (FAAS) determination of peripheral blood of children, calcium, magnesium, copper, iron, zinc and five kinds of elements, from the 2 ml test solution reduced to 0  2? ml, the required analysis of samples of only 20? Μ l. The analysis method is simple, rapid, accurate and reliable. These are as follows. 1 Materials and methods 1  1 Instruments and Materials WFX-110 atomic absorption spectrophotometer (Beijing Rayleigh Analytical Instruments); WN-201-type steady flow pulse micro-injector (Beijing han be produced by); test-specific reagents (Beijing han be produced by); nitric acid ( Top-grade pure). Glass apparatus were required for 10% HNO3 soaked 24? H and above, with de-ionized water rinse clean, dry spare. 1  2 sample collection After disinfection, the taking of children ring finger blood 20? Μ l to join with special detection reagents 2? Ml plastic centrifuge tube, covered with lid immediately shaken, under test. Room temperature conditions, the sample under test does not require the refrigerator; hot weather conditions, the need to test the next day the samples should be placed in the refrigerator to save. 1  3 machines working conditions Lamp Current (mA): Ca 3  0, Mg 10  0, copper 4  0, iron 7  0, zinc