Phosphorylation of separation and enrichment methods in proteomics research progress.docVIP

 PAGE \* MERGEFORMAT 37 Phosphorylation of separation and enrichment methods in proteomics research progress [Abstract] Protein phosphorylation is a reversible post-translational modification, the value in cell differentiation, signal transduction and transcription and translation regulation, protein complex formation, protein degradation, and played an extremely important role. Therefore, the identification of protein phosphorylation as an important post-translational modification of the content. but because of phosphorylation of low abundance proteins, it is difficult to directly detect by mass spectrometry. To solve this problem and improve the MS signal of phosphopeptides response, the need for phosphorylation proteins or phosphopeptides were enriched. This paper systematically describes the phosphorylation of proteomics is widely used in various separation and enrichment of the latest methods to establish the principle, characteristics, progress in applied research, including the antibody enrichment method, specific kinase enrichment, affinity enrichment, chemical modification, a variety of chromatographic separation and enrichment methods and the MALDI target plate enrichment method. [Keywords:] phosphorylation of proteins, separation and enrichment methods, review 1 Introduction Phosphorylation is the most important post-translational modification of protein in the form. According to statistics, the protein in mammalian cells, 1 / 3 can be phosphorylated. Eukaryotic cell protein phosphorylation in the major amino acid tyrosine, serine and Su acid [1]. Among them, the most phosphorylated serine, threonine phosphorylation of the second, the tyrosine phosphorylation of at least three of the ratio of 1800:200:1. The latest experiment to the three identified in eukaryotic cells The percentage was 86.4%, 11.8% and 1.8% [2]. phosphorylated proteins in cell signal transduction, cell differentiation, value-added, cycle control, metabolism, transcripti