Polysaccharide biofilm applications in bone tissue engineering experimental study.doc

 PAGE \* MERGEFORMAT 14 Polysaccharide biofilm applications in bone tissue engineering experimental study Of: Ni Jianguang, Liu Danping, Han Baoqin [Abstract] The purpose of the carrier for bone tissue engineering package polysaccharide outer membrane (PSBM), seed cells in the combined carrier to reduce the time of the spill escaped to verify its feasibility of application of tissue engineering. Methods whole bone marrow obtained by bone marrow stromal stem cells, in vitro amplification, taking third-generation cell cultivation carrier in the porous nano-hydroxyapatite scaffolds - wollastonite, bioactive glass ceramics / chitosan composite scaffolds (AW-MGC/CS, and divided into 2 groups, the experimental group polysaccharide biofilm carrier external physical package, the control group did not wrap polysaccharide biofilm was observed outside the dish on the carrier the number of cell growth, MTT cell viability, and to observe the growth of vector cell adhesion. Results carrier outside the culture plate in experimental group low leakage cells, cells were more leakage: MTT cell viability were detected no significant difference (Pgt; 0.05, the experimental group carrier electron microscopy of cell adhesion is slightly superior growth compared with the control group. Conclusion biofilm polysaccharide in bone tissue engineering of applications. [Keywords:] polysaccharide biofilm bone marrow stromal cells (BMSCs tissue engineering scaffolds Abstract: Objective To pack bone tissue engineering carrier with polysaccharide biomembrane to decrease seed-cells leaking, and to authenticate the feasibility of applying polysaccharide biomembrane in bone tissue engineering. Methods Bone marrow stromal cells were derived through whole-bone-marrow method, and , after amplification in vitro, the cells of the third generation were implanted in AW-MGC/CS. Then the cells were divided into two groups-an experimental group with carrier packed with PSBM and a control group w