PPAR activation on advanced glycation end products induced rat mesangial cell TGF and CTGF mRNA expression.docVIP

 PAGE \* MERGEFORMAT 14 PPAR activation on advanced glycation end products induced rat mesangial cell TGF and CTGF mRNA expression Of: Liu Hui, Miao in Xiaoyan Wei Haifeng Shi Yan Chun-sheng Li Cai Zou Yinggang [Abstract] Objective To observe the peroxisome proliferator-activated receptor (PPAR ) activation on advanced glycation end products (AGEs) induced rat mesangial cell expression of TGF and CTGF mRNA. Methods in vitro cultured normal rat kidney mesangial cells, reverse transcription polymerase chain reaction (RT PCR) detection of different concentrations of AGEs (0 ~ 400 g / ml 1) and different concentrations of PPAP ) activator (rosiglitazone) and PPAR antagonist (GW9662) for AGEs (100 g/ml-1) and TGF induced expression of CTGF mRNA. The results given PPAR activator can significantly reduce AGEs mesangial cells induced by TGF , CTGF mRNA in expression. Conclusion PPAR activator can significantly reduce AGEs on mesangial cell TGF , CTGF mRNA expression effects, thereby improving the glomerular extracellular matrix accumulation in diabetes play a role in kidney protection. [Keywords:] peroxisome proliferator-activated receptor ; diabetic nephropathy; advanced glycation end products; transforming growth factor ; connective tissue growth factor [Abstract] Objective To investigate the role of peroxisome proliferator activated receptor (PPAR) activation on the expressions of TGF and CTGF mRNA in rat mesangial cells extenuation induced by advanced glycation end products (AGEs). Methods Rat mesangial cells were treated with AGE modified bovine serum albumin or native bovine serum albumin. Normal mesangial cells without any treatments were as control. TGF and CTGF mRNA were analyzed by reverse transcriptase polymerase chain reaction (RT PCR). The expressions of TGF and CTGF mRNA in mesangial cell induced by rosiglitazone (0 ~ 10 mol / L) and the inhibitor of PPAR (10 mol / L) were tested with RT PCR. Results PPAR activation relieved TGF a