Rabbit bone marrow-derived mesenchymal stem cells in vitro and biological characteristics of.docVIP

 PAGE \* MERGEFORMAT 17 Rabbit bone marrow-derived mesenchymal stem cells in vitro and biological characteristics of [Abstract] Objective: To further study of bone marrow-derived mesenchymal stem cells (mesenchymal stem cells, MSCs) isolation and culture methods and their biological characteristics, for the use of MSCs as seed cells to treat a variety of diseases, provide an experimental basis. Methods: The density gradient centrifugation, adherent screening isolated and cultured rabbit MSCs. Adhesion rate of vaccination was determined, in the inverted microscope, the cells were observed under the continuous morphological changes, the use of methyl thiazolyl tetrazolium (methyl thiazdyl tetrazolium, MTT) Determination of the growth curve of MSCs and the application of flow cytometry cell surface marker. Results: MSCs adherent after 12 h inoculation rate of 95% or more, the cell doubling time is about 30.8 h, the average passage time of about 5 ~ 7 d; cultured MSCs positive expression of CD29, CD44, CD105, negative expression of CD34. Conclusion: In appropriate experimental conditions, in vitro proliferation of MSCs can survive and maintain the stability of multi-differentiation potential can be used as seed cells for tissue engineering. [Keywords:] bone marrow mesenchymal stem cells; biological characteristics; cell culture The Biological Characteristics and Culture in Vitro of Rabbit Bone Marrow-derived Mesenchymal Stem Cells 〔 Abstract〕 ObjectiveTo further study the isolation and cultivation procedures of mesenchymal stem cells from rabbit in vitro, and explore their biological properties. To build an experimental basis on applying MSCs as seed cells to treat many diseases. Methods MSCs were obtained from tibias by density gradient centrifugation and were tested the rate of adhesion. The configuration was observed under phase-contrast microscope consecutively and the cell growth was measured by MTT method. Cell cycle and surface antigens were detected th