Receptor-type tyrosine kinase RONΔ 160 on NIH-3T3 cell growth and invasive ability of the role of mobility.doc

 PAGE \* MERGEFORMAT 2 Receptor-type tyrosine kinase RONΔ 160 on NIH-3T3 cell growth and invasive ability of the role of mobility [Abstract] Objective To study the receptor-type tyrosine kinase RONΔ 160 on 3T3 cell growth and invasive ability of mobile role. Method of NIH-3T3 cells transfected with plasmid pDR2-RONΔ 160, the establishment of stable expression RONΔ 160 of 3T3-RONΔ 160 cell line; RONΔ 160 immunoprecipitation assay cell tyrosine phosphorylation; inverted microscope observation of cell morphology changes; soft agar was observed RONΔ 160 right NIH-3T3 cell growth capacity; in vitro chemotaxis chamber cross-motion experiments testing RONΔ 160 on NIH-3T3 cells, the role of mobile invasive ability. The results successfully established 3T3-RONΔ 160 cell lines, western blot to prove their stable expression RONΔ 160 protein; RONΔ 160 immunoprecipitation results showed that with its own tyrosine phosphorylation; soft agar growth experiment showed clone cells transfected group number 146.00 ± 3.61, while the non-transfected group and the vector control group were 11.67 ± 1.15 and 12.67 ± 2.52, transfection group and the non-transfected group, vector control group were compared, the differences were statistically significant (t = = 51.60,151.19, P are