Salvia different sources of DNA marker analysis of genetic relationship.docVIP

 PAGE \* MERGEFORMAT 12 Salvia different sources of DNA marker analysis of genetic relationship Author: Xu, Yan-yan, WANG Zheng-Tao, Hu wall [Abstract] Objective To analyze the genetic background of different origin and genetic Salvia relations for the cultivation of medicinal Salvia provide the basis for breeding. Methods RAPD and ISSR markers 4 origin of wild red sage root, the origin of cultivated Salvia 6 a total of 50 samples for analysis, using analysis software to calculate the genetic similarity, the establishment of genetic clustering map. The results of DNA markers were detected 102 loci polymorphic bands ratio (P) of 95.10%, the P value is higher than the cultivation of wild Salvia Salvia; populations Ht the total genetic variation for 0.238 9; UPGMA clustering can be different a good source of Salvia separate areas. Conclusion of different origin exist between the rich genetic diversity of Salvia, Salvia germplasm in the genetic background is more complex, different areas of Salvia has shown clear genetic differentiation, but no correlation with geographic distribution. [Keywords:] Salvia; genetic relationship; DNA marker Abstract: ObjectiveTo study the genetic background and relationship of Salvia miltiorrhizae from different population, and provide reference for the seed selection and cultivation.MethodsGenetic relationship of total 4 wild populations and 6 cultivated populations of S. miltiorrhizae were investigated by ISSR and RAPD analysis, the genetic identity was analyzed by software Popgene 3.2 and the DNA molecular dendrogram was set up by the software NTSYSpc 2.1. ResultsA total of 102 markers with 95.10% polymorphic loci (P) were scored, and the number of P among cultivation population was higher than that of wild population. The Nei’s gene diversity index (Ht) was 0.238 9, and the dendrogram from the clustering of UPGMA showed the good classification of all populations.ConclusionS. miltiorrhizae from different population shows abunda