Saponins Tupistra preliminary anti-tumor effect in vitro experimental study.docVIP

 PAGE \* MERGEFORMAT 14 Saponins Tupistra preliminary anti-tumor effect in vitro experimental study Of: Yang Chunyan, Liuchao Qi, Kun Zou, Wang Jun Zhi, Zhou Yongqin, Liuxiao Qin [Abstract] Objective vitro cell culture techniques to explore the open arrows the cytotoxic activity of saponins and its mechanism for in depth and to provide evidence. Methods Cell viability MTT colorimetric assay, flow cytometry cell cycle and apoptosis . MTT results showed that the total saponin open arrows, 30% and 70% saponins saponin Hela and HepG2 cells was significantly inhibited, compared with the negative control group was significant difference (P lt;0.005. Tupistra saponins, 30 % saponin and 70% saponins and IC50 of cyclophosphamide on Hela cells were 1 372.92,3 138.51,729.72,3 459.08 g / ml, IC50 for HepG2 cells were 1 221.39,2 657.53,562.70,3 668.51g/ml. Flow cytometry showed that the total saponin open arrows, 30% of the total saponin concentration of 2 500g/ml, 70% of the total saponin and cyclophosphamide at a concentration of 1 250 g / ml when the cell cycle of the main resistance to Hela Delay in S phase, and can induce apoptosis of tumor cells. Conclusion Tupistra Hela and HepG2 cells saponins significantly inhibited compared with cyclophosphamide, which are more sensitive to the HepG2 cell line and can be cell cycle arrest in Hela cells S phase and induce apoptosis in Hela cells. [Keywords:] open arrows, saponins, MTT, flow cytometry Tupistra Tupistra chinensis Bak. Department of the lily family Liliaceae Convallarieae Tupistra species, the famous traditional Chinese medicine. Medical historical records, open arrows sweet, slightly bitter flavor, cold, heat treating workers cough, bruises, rheumatism pain, irregular menstruation, uterine bleeding vaginal discharge embolism, including Chinese medicine, irregular menstruation, uterine bleeding and vaginal discharge that is the modern common symptoms of cervical cancer. Tupistra preliminary experiments sho