Sarmentosum alcohol extract of HepG2 human hepatoma cells and its mechanism of inhibition.docVIP

 PAGE \* MERGEFORMAT 16 Sarmentosum alcohol extract of HepG2 human hepatoma cells and its mechanism of inhibition [Abstract] Objective: To observe the ethanol extract of SSB (CCW) on HepG2 human liver cancer cell growth and tumor-related molecular markers. METHODS: MTT method CCW on HepG2 cell proliferation; flow cytometry cell cycle of change; were used RT PCR and immunocytochemistry assay CCW of VEGF, c Myc gene and protein levels. Results: HepG2 cell proliferation in the role of the first CCW 24,48 and 72 h were significantly inhibited; CCW (100,200 g ml-1) can HepG2 cells were arrested in G0/G1 phase; CCW significantly reduced VEGF, c Myc in the expression of mRNA and protein level. Conclusion: CCW on the proliferation of HepG2 cells inhibited the effect, and can prevent the cells into the G2 / M phase, which may be reduced expression of c Myc gene is closely related; CCW can inhibit VEGF secretion in HepG2 cells, initially speculated that CCW has anti-angiogenic effect. [Keywords:] SSB; liver; anti-proliferation; cell cycle; anti-angiogenesis SSB (Sedum sarmentosum Bunge), also known as lying stems Sedum, Crassulaceae, Department of perennial herb found in most parts of the country. Sarmentosum containing mainly SSB glycosides, flavonoids, triterpenes, sterols, alkaloids and sugars. the herbs cool, sweet, light, fresh or dried whole plant medicine, mainly for the treatment of damp heat jaundice, dysuria, sore boils, and can inhibit the inflammatory exudate, reduce liver cell injury to reduce serum aminotransferase levels propyl, treatment of acute and chronic hepatitis and other effects [1]. In recent years, numerous studies show that domestic and foreign, chronic viral hepatitis and liver cancer (hepatocellular carcinoma) is closely related to the occurrence of [23 ], some traditional Chinese herbal medicine in the flavonoids, alkaloids and polysaccharides and other components in the anti-tumor and prolong life in patients and so has a unique bi