SARS coronavirus S protein gene expression and monoclonal antibodies.docVIP

 PAGE \* MERGEFORMAT 14 SARS coronavirus S protein gene expression and monoclonal antibodies Of: PROCEEDINGS Johnson, Xia Mei, Miao Fengqin, Xie D, Zhang Jianqiong [Abstract] Objective: Expression of SARS-CoV S protein fragment, and prepare specific monoclonal antibody (mAb). Methods: Prokaryotic expression of SARS-CoV S protein fragment, cDNA was amplified from the SARS virus S protein (N terminal 205 to 419aa gene and sequenced correctly. This gene fragment was inserted into pQE 30 construct the recombinant plasmid pQE 30 S1m, the recombinant plasmid was transformed into E.coli M15 induced His S1m protein. purified immune BALB / c mice were fused, screening Preparation of specific mAb. RESULTS: The expressed and purified recombinant protein His S1m, get an anti-His S protein mAb1H2.Ig subgroup identified as IgG1a, light chain type. by Western blotting detection, mAb1H2 with the S protein was specific response, but no cross reaction with the His protein. Conclusion: The recombinant S protein was successfully expressed and prepared specific mAb, for the early diagnosis of SARS CoV S protein and further study the function of the foundation. [Keywords:] severe acute respiratory syndrome, SARS coronavirus, S protein, monoclonal antibody Severe acute respiratory syndrome (severe acute respiratory syndrome, SARS) is in November 2002 a new outbreak of infectious disease, infectious disease, strong, short latency, rapid, and high mortality [12]. Although SARS epidemic has been brought under control, but still the possibility of recurrence. Therefore, the development of prevention, diagnosis and treatment methods is important. SARS isolation and identification of pathogens that, SARS-CoV (coronavirus) (SARS CoV) is a new coronavirus. The main structural proteins S, E, M and N, where S protein is a type glycoprotein (gene length 3 768 bp, encoding 1255 amino acids), in function can be divided into S1 and S2 : N-side for the sub-units S1, constitute the mat